Objective: To compare surgical outcomes of single, two- and conventional multi-port laparoscopic and explore the most appropriate surgical method in the treatment of different forms of uterine fibroids. Methods: The data of 66 patients diagnosed with uterine fibroids who underwent single- (22 cases), two- (18 cases) and multi-port laparoscopic myomectomy (26 cases) were retrospectively analyzed. Results: The multi-port group had significantly bigger sizes of uterine fibroids and higher scores of the Visual Analogue Scale than the single-and two-port groups (P<0.05). The single-port group had significantly longer operative times than the other groups (P<0.05). The single-port group had substantially higher postoperative Incision Beauty Scale scores than the multi-port group (P<0.05). However, it was similar to the two-port group in the statistic significance. The single-port group had a significantly higher proportion of anterior wall myoma and fundus myoma than the other two groups (P<0.05). The two-port group had a markedly higher proportion of posterior wall myoma and lateral wall myoma than the single-port group (P<0.05). However, it was similar to the multi-port group in the statistic significance. The multi-port group had a significantly higher proportion of cervical leiomyoma and broad ligament leiomyoma than the single-port group (P<0.05), but was similar to the two-port group in the statistic significance. Conclusion: The laparoscopic single-port myomectomy is a feasible and safe surgical option for anterior wall myoma and fundus myoma. The two-port myomectomy is a better option than the multi-port myomectomy in terms of safety of the procedure, reduction of postoperative pain, and esthetics in posterior wall myoma, lateral wall myoma and other special-part myomas.