TOP2A：肺癌筛选及验证的新型预后标志物
| 作者: |
1杨凯,
1杨明,
1苏学会,
1温辉,
1单娜,
2牛春密
1 秦皇岛市第二医院呼吸科，河北 秦皇岛 066600 2 秦皇岛市第一医院呼吸科，河北 秦皇岛 066000 |
| 通讯: |
杨明
Email: yangkai_6@126.com |
| DOI: | 10.3978/j.issn.2095-6959.2021.09.027 |
| 基金: | 河北省卫生和计划生育委员会科研基金(20191370)。 |
摘要
目的：肺癌是目前对人类健康和生命威胁最大的恶性肿瘤之一，它的发病率高、病死率高，且预后不佳。本研究旨在筛选并验证新的肺癌预后标志物，为肺癌精准治疗提供依据。方法：根据检索GEO数据库中120例肺癌组织和正常组织的表达谱的芯片数据，利用生物信息学和基因组学分析，筛选得到TOP2A基因。通过TCGA数据库对TOP2A基因的表达产物DNA topoisomerase 2-alpha进一步分析，探索了其在肺癌患者中的表达水平，分析该蛋白与预后生存期和肺癌增殖标志物的共表达关系，评价该基因对肺癌细胞系的影响。结果：肺癌组织中TOP2A的表达水平显著高于正常组织，且TOP2A表达量高的患者，其生存期较短。TOP2A表达水平与肺癌增殖能力密切相关。结论：TOP2A基因有望成为新型肺癌预后标志物，为肺癌精准治疗策略选择提供重要依据。
关键词：
肺癌；预后标志物；GEO；TCGA；TOP2A
TOP2A: A new prognostic marker for lung cancer
CorrespondingAuthor: YANG Ming Email: yangkai_6@126.com
DOI: 10.3978/j.issn.2095-6959.2021.09.027
Foundation: This work was supported by Scientific Research Fund of Hebei Health and Family Planning Commission, China (20191370).
Abstract
Objective: Lung cancer is one of the most dangerous malignant tumors with high incidence of mortality and poor prognosis. The purpose of this study is to screen and verify a new prognostic marker of lung cancer, and provide evidence for accurate diagnosis treatment of lung cancer. Methods: In this study, the microarray data of 120 cases of lung cancer and normal tissues in GEO were analyzed, and a potential prognostic marker of lung cancer, TOP2A was obtained. Furthermore, the expression of DNA topoisomerase 2-alpha (coded by TOP2A gene) in patients with lung cancer, the prognostic survival time and the co-expression of proliferation markers of lung cancer were analyzed by TCGA. Moreover, the effect of TOP2A on lung cancer cell line was also evaluated. Results: The results showed that TOP2A was highly expressed in lung cancer tissues, and the survival time of patients with high expression of TOP2A was shorter. TOP2A was closely related to the survival time and proliferation of lung cancer. Conclusion: TOP2A is expected to become a new prognostic marker of lung cancer, which provides a reference for the timely diagnosis and treatment of lung cancer.
Keywords：
lung cancer; prognostic markers; GEO; TCGA; TOP2A