Objective: To explore the influence of perinatal group B streptococcus (GBS) infection screening and sensitive antibiotic treatment on pregnancy outcomes. Methods: A total of 130 pregnant women (35–37 weeks of gestation) who received perinatal GBS screening in our hospital from March 2018 to October 2020 were selected as a positive group. The positive group was divided into group A and group B according to different treatment methods. Group A was given sensitive antibiotics after labor or premature rupture of membranes. Group B was given sensitive antibiotics orally for 7 days after infection, and sensitive antibiotics were given after labor or premature rupture of membranes. Another 120 cases of normal perinatal pregnant women were selected as a negative group. The pregnancy outcomes of pregnant women and newborns were analyzed. Results: GBS was sensitive to penicillin G, cefotaxime, vancomycin, and ampicillin. Compared with the negative group, the positive group had higher incidence of preterm birth, premature rupture of membranes, puerperal infection, fetal distress, jaundice, intrauterine infection, and neonatal pneumonia, the difference was statistically significant (P<0.05). There was no significant difference in the incidence of pregnancy outcomes between group A and group B (P>0.05). Conclusion: GBS infection increases the risk of adverse pregnancy outcomes. It is necessary to pay attention to perinatal GBS screening and preventive treatment of GBS infected patients. The ideal time for treatment is after labor or premature rupture of membranes.