Objective: To explore the effect of psychomotor rehabilitation diagnosis and treatment technology combined with applied behavioral analysis training on the treatment of autistic children. Methods: From March 2019 to October 2019, 40 children with mild to moderate autism who were diagnosed and treated by the Pediatric Children’s Rehabilitation Center of Jiangsu University Affiliated Hospital were divided into an observation group and a control group by random number table method (20 cases in each group). The control group was trained with behavioral analysis method for 6 months, and the observation group was treated with psychomotor rehabilitation diagnosis and treatment technology combined with applied behavioral analysis training for 6 months. The rehabilitation and clinical effects of autistic children were evaluated by Childhood Autism Rating Scale (CARS) and Autism Behavior Checklist (ABC), and Gesell Development Scale (GDS) was used to evaluate the development of autistic children. The effect of two groups of intervention was observed. Results: The CARS scores and ABC scores in the observation group after treatment were lower than those in the control group, and the difference was statistically significant (P<0.05). The scores of the GDS in the observation group were higher than those in the control group after treatment, and the difference was statistically significant (P<0.05). Conclusion: Psycho-motor rehabilitation diagnosis and treatment technology combined with applied behavioral analysis training for autistic children can alleviate their core symptoms of autism, effectively improve the physical application ability of children, and help children recover their dysfunctions. It is of great value to the rehabilitation of autistic children.