Objective: To explore the puncture success rate while using virtual reality technology for intravenous infusion indwelling needle insertion in pediatric patients. Methods: Fifty pediatric patients (aged 4–12 years old) who were classified as American Society of Anesthesiologists (ASA) I–II and received intravenous infusion in the Ninth People’s Hospital between May 2017 and May 2018 were selected. The patients were divided into an experimental group and a control group using a random number table method, with 25 cases in each group. Patients in the experimental group were given a virtual visual aid device during indwelling needle puncture, while the control group received conventional treatment. Results: When children in the experimental group received the conventional method of indwelling needle catheterization, the pain perception degree at T1 and T2 time points was significantly lower than that in the control group (P<0.05). There was no significant difference in the success rate of the first indwelling between the two groups (P>0.05). Conclusion: VR equipment can effectively improve the pain in children with indwelling needle catheterization, and can be popularized in clinical use.