Objective: To compare the effects of two different indwelling methods of thoracic drainage tube on drainage effect, healing, and pain of drainage tube orifice after single port thoracoscopic radical resection of lung cancer. Methods: A total of 80 patients with lung cancer who underwent single port thoracoscopic radical surgery from December 2019 to May 2020 in Anhui Chest Hospital were collected. Single 24F thoracic drainage tube and 24F thoracic drainage tube combined with 8F microcatheter were used respectively. The indwelling time of postoperative drainage tube, postoperative hospital stay, postoperative total drainage volume, intraoperative blood loss, operation time, incision exudation and incision infection, postoperative pain score and healing of drainage tube orifice were observed and analyzed. Results: Compared with the traditional group, the improved group could reduce the indwelling time of drainage tube, reduce the drainage flow, reduce the incision exudation, and shorten the average hospital stay of patients, and the difference was statistically significant (P<0.05). In terms of postoperative pain, the postoperative pain in the improved group was less than that in the traditional group from the second day after operation, and the difference on postoperative day 3 was statistically significant (P<0.05). Conclusion: The improved method of indwelling thoracic drainage tube is feasible, which is conducive to the rapid recovery of patients after operation, and can reduce the incidence of incision leakage and poor healing of drainage tube orifice.