Objective: To investigate the characteristics of Doppler ultrasonography and blood flow spectrum of ectopic pregnancy rupture and its clinical value in differential diagnosis. Methods: The patients with ectopic pregnancy rupture (62 cases) and ovarian corpus luteum rupture (50 cases) confirmed by surgery and pathology were collected and recorded as the ectopic pregnancy rupture group and the ovarian corpus luteum rupture group respectively. Compare the 2 groups of vaginal ultrasound examination, summarize the characteristics of ultrasound and blood flow spectrum of ectopic pregnancy rupture, and observe the value of vaginal ultrasound in the differential diagnosis of ectopic pregnancy rupture. Results: The morphology, boundary, echo and fluid dark area of the adnexal mass in the ruptured ectopic pregnancy group were similar to those in the ruptured corpus luteum group, but the fetal bud, primitive cardiac and pseudocyst of pregnancy could be seen in the former group, which was helpful for the differential diagnosis. Doppler ultrasonography examination showed that the blood flow of ectopic pregnancy rupture group was mainly “dot” and “strip”, and the blood flow spectrum types were diversified. The blood flow performance of ovarian corpus luteum rupture group was mainly “semicircular/annular”, and the blood flow spectrum was extremely low/low resistance type. There was significant difference between the 2 groups (P<0.05). The blood flow parameters, ovarian volume and depth of liquid dark area in ectopic pregnancy rupture group were lower than those in the ovarian corpus luteum rupture group, and the difference was significant (P<0.05). Conclusion: Transvaginal Doppler ultrasonography can provide abundant ultrasonographic features and blood flow spectrum characteristics of ectopic pregnancy rupture, and provide important basis for clinical differential diagnosis of ectopic pregnancy rupture.