Objective: To investigate the protective effect of somatostatin (SS) combined with alanyl glutamine on intestinal function in patients with sepsis. Methods: Seventy patients with sepsis admitted to intensive care unit (ICU) of the Second Affiliated Hospital of Bengbu Medical College from September 2019 to July 2020 were selected as the research objects. The sequential organ failure assessment (SOFA) score was increased by 2 points or more than 2 points compared with the baseline level. The patients were randomly divided into a control group and a combination group with 35 cases in each group. All patients were given basic symptomatic treatment according to the latest sepsis guidelines. The control group was given alanyl glutamine treatment, and the combination group was given SS combined with alanyl glutamine treatment, all of which lasted for 7 days. The levels of serum procalcitonin (PCT), C-reactive protein (CRP), serum bacterial endotoxin (BE), diamine oxidase (DAO) and D-lactic acid (D-Lac) were detected on the day of ICU admission and within 7 days of treatment. The acute physiology and chronic health score (APACHE II), ICU stay and 28-day mortality rate were compared between the two groups. Results: The levels of serum PCT, CRP, BE, DAO, and D-Lac in the two groups after 1 day, 3 days and 7 days of treatment were significantly lower than those on the day of ICU admission (P<0.05), and there was no significant difference between the two groups after 1 d of ICU treatment (P>0.05). After 3 d and 7 d of ICU treatment, the above indexes in the combination group were significantly lower than those in the control group (P<0.05). The APACHE II score of the control group was significantly lower than that of the day of ICU admission after 3 and 7 days of ICU treatment (P<0.05), and the APACHE II score of the combination group was significantly lower than that of the day of ICU admission after 1 day, 3 days and 7 days of ICU treatment (P<0.05), and the APACHE II score and ICU hospitalization time of the combination group were lower than those of the control group after 7 d of ICU treatment (P<0.05), with no significant difference in 28-day mortality between the two groups (P>0.05). Conclusion: SS combined with alanyl glutamine can more fully inhibit the inflammatory reaction and reduce the levels of BE, DAO and D-Lac in patients with sepsis, with a significant protective effect of intestinal function.