长链非编码RNA IGFBP4-1在鼻咽癌中的表达及临床意义
| 作者: |
1张余良,
1邓国庆,
1刘术舟,
2周安燕
1 海南省人民医院(海南医学院附属海南医院)耳鼻咽喉头颈外科，海口 570311 2 海南省人民医院(海南医学院附属海南医院)呼吸与危重症医学科，海口 570311 |
| 通讯: |
周安燕
Email: anni248@163.com |
| DOI: | 10.3978/j.issn.2095-6959.2022.01.006 |
| 基金: | 海南省自然科学基金青年基金(818QN311)。 |
摘要
目的：探讨鼻咽癌(nasopharyngeal carcinoma，NPC)患者癌组织中长链非编码RNA IGFBP4-1(LncRNA IGFBP4-1)的表达及临床意义。方法：选取2014年1月至2014年12月海南省人民医院(海南医学院附属海南医院)耳鼻喉科收治的NPC患者88例为研究组，同期慢性鼻咽炎患者66例为对照组。采用实时定量PCR(quantitative reverse transcription polymerase chain reaction，qRT-PCR)检测两组鼻咽部组织中LncRNA IGFBP4-1表达水平，分析LncRNA IGFBP4-1表达水平与临床病理参数的关系；采用Kaplan-Meier生存曲线及Log-rank分析和比较LncRNA IGFBP4-1高﹑低表达组患者总生存率差异；采用Cox风险比例模型分析影响预后的因素。结果：研究组癌组织中LncRNA IGFBP4-1表达水平高于对照组(6.24±1.09 vs 1.23±0.35，P<0.001)。NPC患者癌组织中LncRNA IGFBP4-1表达水平与性别和年龄无关(P>0.05)，与临床分期、T分级、N分级、远处转移、淋巴结转移﹑复发及EBV DNA相关(P<0.05)。当截断值为4.12时，LncRNA IGFBP4-1区分诊断NPC的曲线下面积(area under the curve，AUC)为0.893 (95%CI：0.763~0.911，P<0.001)， 敏感度81.3%，特异度71.4%。LncRNA IGFBP4-1高表达组5年总生存率低于LncRNA IGFBP4-1低表达组(42.26% vs 57.33%，P<0.05)。多因素分析示，淋巴结转移、临床分期、T分级、LncRNA IGFBP4-1高表达及EBV DNA是影响患者总生存率的独立危险因素。结论：LncRNA IGFBP4-1可很好区分NPC和慢性鼻咽炎患者，LncRNA IGFBP4-1高表达是NPC患者预后不良的分子标志物。
关键词：
长链非编码RNA IGFBP4-1；鼻咽癌；表达；临床意义
Expression of LncRNA IGFBP4-1 in nasopharyngeal carcinoma and its clinical significance
CorrespondingAuthor: ZHOU Anyan Email: anni248@163.com
DOI: 10.3978/j.issn.2095-6959.2022.01.006
Foundation: This work was supported by the Youth Fund Project of Natural Science Foundation of Hainan Province, China (818QN311).
Abstract
Objective: To analyze the expression level and clinical significance of LncRNA IGFBP4-1 in tumor tissues of patients with nasopharyngeal carcinoma (NPC). Methods: A total of 88 patients with NPC treated in our hospital from January 2014 to December 2014 were selected as the study group; 66 patients in the same period with chronic nasopharyngitis were selected as the control group. The expression level of LncRNA IGFBP4-1 in nasopharynx tissues of the two groups was detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). The relationship between LncRNA IGFBP4-1 expression level and clinicopathological parameters was analyzed. Kaplan-Meier survival curve and Log-rank were used to analyze and compare the overall survival rate of patients with high and low expression of LncRNA IGFBP4-1. Cox risk ratio model was used to analyze the univariate and multivariate prognostic factors. Results: The expression of LncRNA IGFBP4-1 in the study group was higher than that in the control group (6.24±1.09 vs 1.23±0.35, P < 0.001). The expression of LncRNA IGFBP4-1 was not correlated with gender and age (P>0.05), but correlated with clinical stage, T grade, N grade, distant metastasis, lymph node metastasis, recurrence and EBV DNA (P<0.05). When the cut-off value was 4.12, the area under the curve (AUC) of LncRNA IGFBP4-1 was 0.893 (95%CI: 0.763–0.911, P<0.001), the sensitivity and specificity were 81.3% and 71.4%, respectively. The 5-year overall survival rate of patients with high LncRNA IGFBP4-1 expression was lower than that of patients with low LncRNA IGFBP4-1 expression (42.26% vs 57.33%, P<0.05). Multivariate analysis showed that lymph node metastasis, clinical stage, T grade and high expression of LncRNA IGFBP4-1 and EBV DNA were independent risk factors affecting the overall survival rate of patients. Conclusion: LncRNA IGFBP4-1 can distinguish well NPC patients from patients with chronic nasopharyngitis. High expression of LncRNA IGFBP4-1 is a molecular marker of poor prognosis in NPC patients.
Keywords：
long non-coding RNA IGFBP4-1; nasopharyngeal carcinoma; expression; clinical significance