Objective: To investigate the department distribution and antibiotics resistance of Klebsiella pneumoniae (KP) in a tertiary hospital located in Hohhot, as well as providing evidence for clinical decisions for KP infected patients. Methods: In this retrospective analysis, we collected the clinical and antibiotics resistance characteristics of 2 975 strains of KP isolated from the Affiliated Hospital of Inner Mongolia Medical University from January 2016 to December 2019. The VITEK 2 Compact automated analyzer was used for antibiotics susceptibility testing. The drug susceptibility results were interpreted in accordance with the American Clinical and Laboratory Standards Institute (CLSI) M300-S23 guideline. The collected data were analyzed by WHONET 5.6 statistical software. Results: Among the 2 975 non-duplicated clinical isolates, the main sources of strain specimens were sputum (61.24%, 1 822/2 975) and urine (26.82%, 798/2 975). The clinical departments were mainly distributed in the Department of Respirology (14.22%, 423/2 975), Surgery (11.19%, 333/2 975) and Intensive Care Unit (9.34%, 278/2 975). The isolated KP were mainly resistant to ciprofloxacin, ceftriaxone, and cefazolin. The resistance rate to ceftazidime, piperacillin/tazobactam, meropenem and imipenem were increased from 2016 to 2019. Conclusion: KP strains have shown multi-drug resistance or even extensive drug resistance trends. Clinicians should pay special attention to carbapenem-resistant isolates. The treatment approach for KP infection should be based on clinical characteristics, especially the result of antibiotics resistance testing.