Objective: To study the value of serum amyloid A (SAA) combined with C-reactive protein (CRP) in the early diagnosis of influenza A H1N1 flu, and to provide clinical guidance for early diagnosis of patients. Methods: A total of 80 children with influenza A received by our hospital from January 2019 to January 2020 were included as research objects and were analyzed retrospectively. Among them, children diagnosed by serum CRP were identified as the control group and children diagnosed by serum CRP combined with SAA were included as the experimental group. The levels of CRP and WBC were recorded, and the reference value of CRP diagnosis and CRP combined with SAA diagnosis in children with influenza A was analyzed and so was the correlation between serum SAA and CRP. Results: The serum CRP index of the experimental group was significantly higher than that of the control group (P<0.05), but there was no significant difference in WBC count between the two groups (P>0.05); the best cutoff value of serum CRP was 13.2 mg/L, while the best cutoff value of CRP combined with SAA was 10.4 mg/L. AUC of CRP combined with SAA was 0.905, which was significantly higher than that of CRP (0.743) alone (P<0.05); the sensitivity and specificity of CRP combined with SAA were 88.7% and 83.5%, respectively, which were significantly higher than those of CRP alone (75.8% and 73.7%, P<0.05). There was a positive correlation between SAA and CRP in the experimental group (r=0.821, P<0.05). Conclusion: The levels of serum amyloid A and C-reactive protein are significantly increased in children with influenza A. The combination of serum amyloid A and CRP can provide guidance and is recommended for early diagnosis of influenza A H1N1 flu.