Objective: To evaluate the performance of automatic nucleic acid extractor and fluorescence quantitative polymerase chain reaction (PCR) analysis system Anadas9850 for detection of hepatitis B virus nucleic acid (HBV-DNA). Methods: According to the guidelines approved by the Clinical and Laboratory Standards Institution (CLSI), the performance verification of the automatic nucleic acid extractor and fluorescence PCR analysis system Anadas9850 for detection of HBV-DNA kit was carried out, including precision, accuracy, linear test, minimum detection range and anti-interference ability. Results: In terms of precision, the coefficient of variations (CVs) of high and low intra-batch precision were 1.71% and 2.64% respectively. The high and low CVs of inter-batch precision were 2.40% and 2.61%, respectively. In terms of accuracy, the difference between the logarithmic value of the test result of the sample and the quality control target value was ±0.4 in log value. It had good linearity in the gradient range of 50 to 5.0×10⁸ U/mL. The linear regression equation was Y = 0.9385X ± 0.2552, R²=0.9986. The minimum detection rate of the experimental results was 100%, which met the detection requirements. In terms of anti-interference ability, hemolysis, hyperlipidemia, and high bilirubin had no effect on the test results. Conclusion: The performance of automatic nucleic acid extractor and fluorescence PCR analysis system Anadas9850 for detection of hHBV-DNA meets the requirements of clinical detection and can be used in clinical detection.