斑马鱼癌症模型在肿瘤精准医疗中的研究进展
| 作者: |
1张淑慧,
2赵承湉,
1冯喜增
1 南开大学药物化学生物学国家重点实验室，生物活性材料教育部重点实验室，生命科学学院，天津 300071 2 华南理工大学生物科学与工程学院，广州 510006 |
| 通讯: |
冯喜增
Email: xzfeng@nankai.edu.cn |
| DOI: | 10.3978/j.issn.2095-6959.2021.09.034 |
| 基金: | 国家重点研究与发展项目(2019YFB1309704)。 |
摘要
在肿瘤精准医疗中，主要采用两种方法来预测临床相关的肿瘤行为(如治疗响应和药物耐药性的出现)：基于患者样本基因组、转录组、表观基因组和/或蛋白质组学进行分析推断，以及在个体化癌症患者“替身”中进行表型研究。后一种方法依赖于小鼠体内异种移植模型和体外类器官癌症模型或2D细胞培养。患者源性异种移植免疫受损成年斑马鱼品系的建立，以及基于斑马鱼幼鱼异种移植模型药物反应表型测试的第1项临床试验的开展，使斑马鱼这种小脊椎动物应用于精准医疗领域的前沿。
关键词：
肿瘤精准医疗；斑马鱼；转基因癌症模型；癌症异种移植模型
Research progress of zebrafish patient avatars in precision oncology
CorrespondingAuthor: FENG Xizeng Email: xzfeng@nankai.edu.cn
DOI: 10.3978/j.issn.2095-6959.2021.09.034
Foundation: This work was supported by the National Key R&D Program of China (2019YFB1309704).
Abstract
In precision cancer medicine, two major approaches are used to predict clinically relevant tumor behaviors (such as treatment response and the emergence of drug resistance): analysis and extrapolation based on genome, transcriptome, epigenomics, and/or proteomic of patient samples, and phenotypic studies were performed in individualized models of patients with a cancer. The latter strategy relies on xenograft models in mice and the cancer models of organoids or 2D cell culture in vitro. The establishment of immunocompromised adult zebrafish models of patient-derived xenografts and the development of the first clinical trial based on the drug response test of zebrafish larvae xenografts make this vertebrate to be applied in the frontier of precision medicine.
Keywords：
precision oncology; zebrafish; transgenic cancer models; cancer xenotransplantation model