Objective: To investigate the effects of ultrasound and anti-itch scar softening ointment in the treatment of burn scar pruritus and the influence on the prognosis. Methods: A total of 80 patients who had pruritus after treatment for burn scars in the hospital between February 2016 and February 2018 were selected as the subjects. They were randomly divided into two groups with 40 patients in each group. Both groups were treated with anti-itch scar softening ointment, and the combined group were additionally treated with ultrasound. The degree of pruritus, pruritus scores and levels of scar related factors before and after the treatment, treatment effects and prognosis of the two groups were compared. Results: After the treatment, the degree of pruritus in both groups was significantly relieved (Hc=52.771, 31.782, P<0.05), and it was milder in the combined group than the control group (Hc=4.632, P<0.05). After the treatment, the degree, location and frequency of pruritus and effect on sleep were significantly improved (P<0.05), and they were better in the combined group than the control group (P<0.05). After treatment, levels of serum epidermal growth factor (epidermal growth factor, EGF), transforming growth factor (transforming growth factor, TGF-β1) and substance P in both groups were decreased significantly (P<0.05), and the levels in combined group were lower than those in the control group (P<0.05). The total response rate and satisfaction rate with appearance of the combined group (97.50% and 92.50%) were higher than those of the control group (80.00% and 70.00%), while the recurrence rate of pruritus (20.00%) was lower than the control group (47.50%, P<0.05). Conclusion: Ultrasound combined with anti-itch scar softening ointment is effective in the treatment of burn scar pruritus. It can significantly reduce the levels of related factors and improve the prognosis of patients.