一种简便的大鼠II度烫伤模型制备方法
| 作者: |
1丁毅,
2吾拉尔·阿德力,
1李敏,
1冯树梅
1 新疆医科大学基础医学院组织学与胚胎学教研室，乌鲁木齐 830011 2 新疆医科大学第一临床学院，乌鲁木齐 830011 |
| 通讯: |
冯树梅
Email: 87391167@qq.com |
| DOI: | 10.3978/j.issn.2095-6959.2021.03.004 |
| 基金: | 国家自然科学基金(81660324，81660521)；新疆医科大学教育教学研究与改革项目(YG2019053)。 |
摘要
目的：观察砝码加热制备Wistar大鼠烫伤模型的效果。方法：以纱布烫伤作为对照，评估砝码烫伤制备大鼠烫伤模型的效果，并分别从肉眼和镜下观察了不同制备方法下大鼠皮肤创面的形态结构改变。结果：在温度100 ℃、烫伤时间15 s的条件下，分别用纱布和砝码烫伤Wistar大鼠。纱布烫伤模型组符合I度烫伤病理改变，而砝码烫伤模型组符合II度烫伤病理改变。结论：使用砝码进行大鼠烫伤模型的制备具有烫伤面积可控，重复性好、稳定性强，操作简便可行等优点，适宜作为II度烫伤模型的简易制备方法，为烫伤的发生和治疗研究奠定基础。
关键词：
大鼠；烫伤；模型；砝码烫伤；纱布烫伤
A simple preparation method of II degree scald model in rats
CorrespondingAuthor: FENG Shumei Email: 87391167@qq.com
DOI: 10.3978/j.issn.2095-6959.2021.03.004
Foundation: This work was supported by the National Natural Science Foundation of China (81660324, 81660521) and the Educational Research and Reform Project of Xinjiang Medical University (YG2019053), China.
Abstract
Objective: To observe the effect of using heating weights in a Wistar rat scald model. Methods: Gauze burns were used as a control to evaluate the effect of the method for preparing rat scald models by weight burns, and the morphological changes of rat skin wounds under different preparation methods were observed from eyes and microscope. Results: Wistar rats were burned with gauze and weights at a temperature of 100 ℃ and a burn time of 15 s, respectively. The pathological changes in the gauze scald model group and the weight scald model group were consistent with first- and second-degree burns, respectively. Conclusion: The preparation of rat scald model using weights has the advantages of controllable scald area, good repeatability, strong stability, easy operation, etc. It is suitable as a simple method for preparing a II degree scald model and lays the foundation for the research on the occurrence and treatment of scald.
Keywords：
rat; scald; model; scald with weight; scald with gauze