Objective: To investigate the clinical risk factors and molecular epidemiological characteristics of carbapenem-resistant hypervirulent K. pneumoniae (CR-hvKP) infection, providing theoretical guidance for clinical prevention and treatment of CR-hvKP strains. Methods: The CR-hvKP strain was firstly isolated from the Affiliated Hospital of Xuzhou Medical University from January 1, 2017 to May 31, 2019. The relevant clinical information was collected, and the strain identification was performed by mass spectrometry. VITEK2 Compact automatic bacteria analyzer was used for drug sensitivity test, multi-site sequence analysis (MLST) for homologous typing, and PCR for common carbapenem resistance genes (KPC, SME, GES, VIM, IMP, OXA-48), serum capsular gene (K1, K2, K5, K20, K54) and virulence genes (rmpA, magA, aerobactin, Iron) detection. Results: A total of 26 carbapenem-resistant and highly toxic K. pneumoniae were collected. Most of the patients suffered from underlying diseases (hypertension, diabetes and other chronic diseases). They have recently undergone invasive procedures or treatments and used high-grade antibacterial drugs. The wire drawing experiments were all positive; the MLST classification was mainly ST11 (73.1%); carbapenem gene was mainly KPC-2 (96.2%); serum capsule type was mainly K1 and K2, mostly carrying rmpA, aerobactin virulence gene. Conclusion: CR-hvKP has the characteristics of both hvKP and CRKP: high drug resistance, high invasiveness and high transmission. This bacterial infection is serious, difficult to treat, and easily causes nosocomial infection. Preventive measures should be taken early, like rational use of antibiotics, adherence to the principle of sterility, and timely isolation of patients to block the outbreak of CR-hvKP in hospitals.