Objective: To investigate the effect and mechanism of miR-144-3p on the growth and invasion of ovarian cancer. Methods: The expression of miR-144-3p and SGK3 mRNA in normal ovarian epithelial cells and ovarian cancer cells was detected by RT-qPCR, and the targeting relationship was detected by dual luciferase assay. The ovarian cancer SKOV3 cells were divided into a control group, a mimic-NC group, a miR-144-3p mimic group, a pc-SGK3 group, and a miR-144-3p+ SGK3 group. Cell proliferation was detected by CCK-8 method, cell growth was detected by colony formation assay, cell cycle and apoptosis were detected by flow, cell invasion was detected by transwell assay, and the expression levels of p-MST, p-LATS, p-YAP, MST, LATS and YAP were detected by Western blotting. The transplanted tumor was constructed by subcutaneous injection of SKOV3 cell suspension into the hind limb of nude mice. The volume of the transplanted tumor was detected weekly. On the 30th day, the nude mice were sacrificed by cervical dislocation. The subcutaneous tumor was completely removed and the electronic balance was weighed. The expression levels of SGK3, p-MST, p-LATS, p-YAP, MST, LATS, and YAP proteins were detected by Western blotting. Results: MiR-144-3p was down-regulated in ovarian cancer cells, while SGK3 was highly expressed; miR-144-3p was targeted to negatively regulate SGK3; miR-144-3p overexpression significantly reduced ovarian cancer cell proliferation, reduced the number of ovarian cancer cell clones per field of vision, shortened the cell cycle, increased the rate of apoptosis, reduced the number of invading cells, and up-regulated p-MST/MST, p-LATS/LATS, and p-YAP/YAP protein expression (P<0.01), the addition of SGK3 high expression and Hippo signaling pathway inhibitor XMU-MP-1 reversed the above reaction. Conclusion: MiR-144-3p inhibits the growth and invasion of ovarian cancer by targeting SGK3 via Hippo signaling pathway.