Objective: To investigate the detailed measures of refined process management in the hospital operating room, and analyze the effect of control infection. Methods: The research started from January, 2019 and ended in December, 2019. Refined process management in the operating room of No. 904 Hospital, Logistics Support Force of People’s Liberation Army of China was carried out and as an observation group, compared with the control group, which is under the regular management of the time from January, 2018 to December, 2018. The management quality was evaluated and analyzed, and the passing rate of sample collection was calculated. Results: In the observation group, quality of goods management score (95.58±1.03), quality of human management score (96.89±1.05), quality of environmental management score (94.35±2.77) higher than that of the control group (P<0.05). Moreover, the passing rate of the observation group sample was higher than that of the control group (P<0.05). Conclusion: Refined process management can effectively enhance the quality of hospital management, controlling the infection and is worth promoting in clinical application.