Objective: To explore the application value of perioperative nursing based on three-dimensional electroanatomic mapping system (CARTO) in radiofrequency ablation of atrial fibrillation (AF) and to provide guidance for patients’ diagnosis and treatment. Methods: A total of 70 patients who underwent radiofrequency surgery for AF in Wanbei Coal Power Group General Hospital from January 2017 to July 2019 were selected as study subjects. All patients underwent CARTO-guided radiofrequency ablation of AF and analyzed their related nursing interventions. Results: All patients successfully completed the operation. The operation time was (214.84±55.79) min. The total effective rate was 97.14%, and the nursing satisfaction was 98.57%. Among them, AF still occurred in 5 patients with persistent AF, and sinus rhythm was restored after immediate electrical conversion. There were no complications during the operation, no other serious complications occurred after 3 months. Follow-up was 3 to 12 months, one case of recurrent AF showed a recurrence rate of 97.91%, two cases of persistent AF recurred and the rate of success was 90.91%. Conclusion: Effective nursing measures have significant effects in the application of CARTO three-dimensional radiofrequency ablation of AF. The effective nursing measures can be developed before, during and after surgery to reduce the psychological pressure of patients before and after surgery and improve the success of surgery rate, reducing postoperative recurrence, being safer and more effective, and worthy of clinical promotion and application.