To investigate the clinicopathological features and biological behavior of columnar cell variant of thyroid papillary carcinoma (CCV-PTC), the clinical data of 4 cases of CCV-PTC were collected and analyzed. The pathological features and immunophenotypes of CCV-PTC were observed. Two female and two male among the four patients, with an average age of 63 years (range, 54–81 years). Peripheral striated muscle invasion and lung metastasis were found in the male patient. Capsule invasion and lymph node metastasis in the deep inferior and central region of the neck were found in another male patient. And the female patient was accompanied by poorly differentiated carcinoma. The histologic features showed the tumor cells were columnar and pseudostratified, and occasional showed supranuclear/subnuclear vacuolization, similar to the secretory form of the endometrium. The immunohistochemistry of the columnar cells showed positive of TTF-1, CK19, TG and BRAFV600E. CCV-PTC is a rare subtype of papillary thyroid carcinoma, which is more invasive than classical papillary thyroid carcinoma. It can be diagnosed according to its histologic characteristics and immunophenotypes.