番红花素促进烧伤大鼠的创面愈合与减轻疼痛
| 作者: |
1郭洪耀,
1林斌,
1沙树奎
1 南阳市中医院烧伤整形科，河南 南阳 473000 |
| 通讯: |
郭洪耀
Email: ghyghy212@126.com |
| DOI: | 10.3978/j.issn.2095-6959.2020.03.006 |
摘要
目的：研究番红花素对烧伤大鼠的创面愈合与疼痛影响。方法：将80只大鼠随机分成假烧伤组(n=16)，烧伤模型组(n=16)，烧伤+10 mg/kg番红花素组(n=16)，烧伤+50 mg/kg番红花素组(n=16)，烧伤+200 mg/kg番红花素组(n=16)。使用在干浴中预热至165 ℃的铜块(2 cm×2 cm，
150 g)构建大鼠皮肤全厚度烧伤。采用HE染色观察组织学恢复情况，检测不同处理组大鼠的机械阈值和热阈值，蛋白质印迹法和ELISA方法检测创面皮肤组织中诱导型一氧化氮合酶(iNOS)和炎性因子IL-6和TNF-α的表达。结果：番红花素的应用可以促进创面皮肤伤口愈合并减轻疼痛。且番红花素抑制创面皮肤组织中iNOS的表达和炎性因子IL-6和TNF-α水平。这一疗效在应用200 mg/kg的剂量时最为明显。结论：番红花素可以促进烧伤大鼠的创面愈合并减轻疼痛。
关键词：
番红花素；烧伤；创面愈合
150 g)构建大鼠皮肤全厚度烧伤。采用HE染色观察组织学恢复情况，检测不同处理组大鼠的机械阈值和热阈值，蛋白质印迹法和ELISA方法检测创面皮肤组织中诱导型一氧化氮合酶(iNOS)和炎性因子IL-6和TNF-α的表达。结果：番红花素的应用可以促进创面皮肤伤口愈合并减轻疼痛。且番红花素抑制创面皮肤组织中iNOS的表达和炎性因子IL-6和TNF-α水平。这一疗效在应用200 mg/kg的剂量时最为明显。结论：番红花素可以促进烧伤大鼠的创面愈合并减轻疼痛。
External application of crocetin promotes wound healing and reduces pain in burned rats
CorrespondingAuthor: GUO Hongyao Email: ghyghy212@126.com
DOI: 10.3978/j.issn.2095-6959.2020.03.006
Abstract
Objective: To investigate the effects of crocetin on wound healing and pain in burned rats. Methods: Eighty rats were randomly divided into 5 groups: the sham group (n=16), the burn model group (n=16), the burn+10 mg/kg crocetin group (n=16), the burn + 50 mg/kg crocetin group (n=16), and the burn + 200 mg/kg crocetin group (n=16). The 165 ℃ copper block (2 cm×2 cm, 150 g) was used to create full-thickness burn injuries in the rats. The histological recovery was observed by HE staining. The mechanical threshold and thermal threshold of rats in different treatment groups were detected. The levels of inducible nitric oxide synthase (iNOS) and inflammatory factors IL-6 and TNF-α in wound skin tissues were detected by Western blot and ELISA assay. Results: Crocetin promoted wound healing and reduced pain. Furthermore, crocetin inhibited the expression of iNOS and reduced the levels of inflammatory factors IL-6 and TNF-α in wound skin tissues. This effect was most pronounced at doses of 200 mg/kg. Conclusion: Crocetin can promote wound healing and reduce pain in burned rats.
Keywords：
crocetin; burn; wound healing