Caveolin-1基因的甲基化状态与食管胃结合部腺癌中Caveolin-1 mRNA，Caveolin-1蛋白及Her-2蛋白表达的相关性
| 作者: |
1石晓鹏,
1尹颖,
1张金平,
1孔易
1 武安市第一人民医院病理科，河北 武安 056300 |
| 通讯: |
孔易
Email: kongyi1111@163.com |
| DOI: | 10.3978/j.issn.2095-6959.2020.02.004 |
| 基金: | 河北省卫生厅研究与发展项目(20171168)。 |
摘要
目的：检测食管胃结合部腺癌(adenocarcinomas of the esophagogastric junction，AEG)及远端正常组织(距癌灶>10 cm)中Caveolin-1的甲基化水平，研究该基因甲基化对其mRNA、蛋白质及人类表皮生长因子受体2(human epidermal growth factor receptor-2，Her-2)蛋白在AEG中表达的相关性。方法：采用甲基化特异性PCR(methylation-specific PCR，MSP)法检测67例食管胃结合部腺癌及远端正常组织中Caveolin-1的甲基化状态；采用RT-PCR法检测食管胃结合部腺癌及远端正常组织中Caveolin-1 mRNA表达情况；应用免疫组织化学法检测Caveolin-1蛋白及Her-2蛋白的表达情况；进一步分析Caveolin-1的甲基化状态与其mRNA、蛋白及Her-2蛋白的表达的相关性。结果：在食管胃结合部腺癌中，Caveolin-1的甲基化表达率为28.4%(19/67)，远端正常组织中未发现该基因的甲基化现象，差异有统计学意义(P<0.01)；癌组织中Caveolin-1的甲基化水平与患者的肿瘤大小、分化程度及淋巴结转移方面有关，差异有统计学意义(P<0.05)；而与性别、年龄、有无脉管癌栓及临床分期无关，差异无统计学意义(P>0.05)；癌组织中Caveolin-1 mRNA及蛋白的阳性表达率分别为46.3%和41.8%，均明显低于远端正常组织，差异有统计学意义(P<0.01)。癌组织中Caveolin-1蛋白及Her-2蛋白的阳性表达率分别为41.8% 和56.7%，均与Caveolin-1的甲基化状态相关，差异有统计学意义(P<0.05)。结论：Caveolin-1甲基化可能参与了肿瘤的表达，并且Caveolin-1甲基化可能与食管胃结合部腺癌中Caveolin-1 mRNA，Caveolin-1蛋白及Her-2蛋白的表达相关，在食管胃结合部腺癌的发生及发展中发挥着重要的作用。
关键词：
食管胃结合部腺癌；Caveolin-1甲基化；Caveolin-1蛋白；Her-2蛋白
Correlation between methylation of Caveolin-1 and expression of Caveolin-1 mRNA, Caveolin-1 protein and Her-2 protein in human adenocarcinomas of the esophagogastric junction
CorrespondingAuthor: KONG Yi Email: kongyi1111@163.com
DOI: 10.3978/j.issn.2095-6959.2020.02.004
Foundation: This work was supported by Research and Development Project, Health Commission of Hebei Province，China (20171168)
Abstract
Objective: To investigate the methylation level of Caveolin-1 in human adenocarcinomas of the esophagogastric junction (AEG) and the distal normal tissue from carcinoma (>10 cm), and to study the expression of Caveolin-1 mRNA, Caveolin-1 protein and Her-2 protein in AEG and their relationships with the methylation of Caveolin-1. Methods: Methylation-specific PCR (MSP) method was used to detect the methylation status of Caveolin-1 in 67 patients with AEG and distal normal tissue from carcinoma; the expression of Caveolin-1 mRNA was detected in AEG and the distal normal tissue from carcinoma by RT-PCR; the expression of Caveolin-1 protein and Her-2 protein was detected by immunohistochemical method; the correlation between the methylation of Caveolin-1 and the expression of Caveolin-1 mRNA, Caveolin-1 protein and Her-2 protein was further analyzed. Results: Methylation of Caveolin-1 was expressed as 28.4% (19/67) in AEG, and no methylation of this gene was found in the distal normal tissue from carcinoma, and the difference was statistically significant (P<0.01). Methylation level of Caveolin-1 in carcinoma tissues was related to tumor size, differentiated degree and lymph node metastasis of patients, and the difference was statistically significant (P<0.05), but there was not related to gender, age, presence of vascular carcinoma thrombus and clinical stages, and the difference was not statistically significant (P>0.05). The positive expression rate of Caveolin-1 mRNA and the protein in carcinoma tissues were 46.3% and 41.8%, which were both lower than the expression rate of that in the distal normal tissue from carcinoma, and the difference was statistically significant (P<0.01). The positive expression rate of Caveolin-1 protein and Her-2 protein in carcinoma tissues were 41.8% and 56.7% , which both related to the methylation state of Caveolin-1, and the difference was statistically significant (P<0.05). Conclusion: Methylation of Caveolin-1 may be involved in the expression of carcinoma, and may be associated with the expression of Caveolin-1 mRNA, Caveolin-1 protein and Her-2 protein in AEG, which may play an important role in the occurrence and development of AEG.
Keywords：
adenocarcinomas of the esophagogastric junction; methylation of Caveolin-1; Caveolin-1 protein; Her-2 protein