Objective: To investigate the effects of miR-135 expression in oocytes on embryonic development. Methods: Real-time quantitative polymerase chain reaction (real-time PCR) was used to detect the expression of miR-135 in blastocysts with different developmental quality. Then, the oocytes were injected with miR-135 inhibitor before in vitro fertilization, the composition ratio of different developmental quality was analyzed. The number of 2 cell stage, 4 cell stage, morula stage and blastocyst stage during the development of preimplantation embryos was detected. The expression and distribution of IGF1R in blastocysts was observed by fluorescence staining. After the blastocyst was implanted into mice, the length of the crown and hip of the developing fetus was observed. Results: MiR-135 was highly expressed in high quality blastocysts. After the oocytes were injected with miR-135 inhibitor and then fertilized in vitro, the proportion of high-quality blastocysts was decreased and preimplantation embryonic development became retardation. Microscopic observation showed no significant change in the expression of IGF1R in blastocysts, but the distribution pattern was changed, and the expression was concentrated on the basement membrane. After implantation, the length of the crown and buttock in fetal mice became shorter. Conclusion: Inhibition of miR-135 expression in mouse oocytes inhibits embryonic development.