Micro146b在甲状腺乳头状癌中的表达及其对甲状腺乳头状癌侵袭迁移的影响
| 作者: |
1龚麒麟,
1叶钦,
1刘辉
1 福建省肿瘤医院，福建医科大学附属肿瘤医院头颈外科，福州 350014 |
| 通讯: |
刘辉
Email: liuhuifj@sina.com |
| DOI: | 10.3978/j.issn.2095-6959.2019.11.006 |
| 基金: | 福建省科技厅自然科学基金(2017J01258)；福建省卫生健康科研人才培养项目(2016-1-13)。 |
摘要
目的：探讨miR-146b在甲状腺乳头状癌中的表达及其对甲状腺乳头状癌侵袭转移的影响。方法：体外培养人甲状腺乳头状癌TPC-1细胞并转染miR-146b，分为4组：miR-146b抑制组及抑制对照组、miR-146b转染组及转染对照组。分别通过qRT-PCR检测miR-146b的表达，CCK-8法检测细胞增殖，Transwell法检测细胞侵袭能力，划痕法检测细胞的迁移能力。结果：MiR-146b在甲状腺乳头状癌TPC-1细胞中高表达(P<0.01)。CCK-8法检测发现miR-146b抑制组TPC-1细胞活力与抑制对照组相比显著降低(P<0.01)，而miR-146b转染组细胞活力与转染对照组相比显著提高(P<0.01)。Transwell法检测发现抑制miR-146b后细胞的穿膜数明显减少(P<0.01)，而过表达miR-146b后细胞的穿膜数明显增加(P<0.01)。划痕法检测发现miR-146b抑制组的迁移距离与抑制对照组比显著扩大(P<0.01)，而miR-146b转染组的迁移距离与对照组比显著缩小(P<0.01)。结论：甲状腺乳头状癌TPC-1细胞中存在miR-146b的高表达。miR-146b的高表达可促进TPC-1细胞的增殖、侵袭及迁移。
关键词：
微小RNA-146b；甲状腺乳头状癌；侵袭；迁移
Expression of miR-146b in thyroid cancer and its role in invasion and migration of thyroid cancer cells
CorrespondingAuthor: LIU Hui Email: liuhuifj@sina.com
DOI: 10.3978/j.issn.2095-6959.2019.11.006
Foundation: This work was supported by the Natural Science Foundation of Fujian Province (2017J01258) and Fujian Provincial Health Technology Project (2016-1-13), China.
Abstract
Objective: To investigate the expression level of micro RNA-146b in thyroid cancer cell lines and its impact on thyroid cancer invasion and metastasis. Methods: MiR-146b was transfected into human thyroid cancer cells TPC-1 in vitro. All cells were divided into an inhibited miR-146b group, an inhibitor negative control group, a transfected miR-146b group, and a negative control group. MiR-146b expression was detected by qRT-PCR in thyroid cancer cell lines (TPC-1) and negative control cell lines. The characteristics of invasion and migration of the transfected MiR-146b cells were examined by transwell assay. The expression of miR-146b was detected by qRT-PCR, cell proliferation was determined by CCK-8 assay. Migration rate was measured by wound healing assay. Results: MiR-146b was highly expression in thyroid cancer cells TPC-1 (P<0.01). CCK-8 assay found that the cell viability in the miR-146b inhibitor group were significantly lower than that in the inhibitor NC group (P<0.01), while that in the miR-146b mimics group were significantly higher than that in the NC group (P<0.01). Transwell assay found that the number of cells through membranes was decreased evidently in the miR-146b inhibitor group (P<0.01), while that was increased evidently in the miR-146b mimics group (P<0.01). The wound healing assay found that the migration distance of miR-146b inhibitor group was farther than that in the inhibitor NC group (P<0.01), while that of the miR-146 mimics group was shorter than that in the NC group (P<0.01). Conclusion: MiR-146b has high expression in thyroid cancer cells TPC-1. The overexpression miR-146b can promote cell proliferation, invasion, and migration.
Keywords：
microRNA; papillary thyroid carcinoma; invasion; migration