维生素E对CBA小鼠年龄相关性听力损失的保护作用及其机制
| 作者: |
1李兴程,
2周涛
1 郑州大学第一附属医院耳鼻喉医院耳科，郑州 450052 2 华中科技大学同济医学院附属协和医院耳鼻喉科，武汉 430022 |
| 通讯: |
李兴程
Email: fcclixc@zzu.edu.cn |
| DOI: | 10.3978/j.issn.2095-6959.2019.11.003 |
摘要
目的：探讨维生素E(Vitamin E)能否改善年龄相关性CBA小鼠听力损失及其机制。方法：CBA小鼠分为对照组与Vitamin E干预组，其中Vitamin E干预组给予含Vitamin E的饲料，对照组给予正常饲料。通过检测小鼠听觉脑干反射阈值(auditory brainstem response，ABR)评估小鼠听力情况；用荧光素488标记的鬼笔环肽计算外毛细胞个数；扫描电镜检测外毛细胞静纤毛状态；检测耳蜗超氧化物歧化酶(superoxide dismutase，SOD)和谷胱甘肽过氧化物酶(glutathione peroxidase，GPx)活性水平；超氧阴离子荧光探针检测外毛细胞的超氧阴离子水平；免疫荧光检测外毛细胞cleaved caspase-3阳性细胞数。结果：CBA小鼠10月龄时，对照组有明显的听力损失。与对照组相比，Vitamin E干预组小鼠ABR阈值降低，外毛细胞数增加，静纤毛结构病变明显缓解，耳蜗SOD及GPx活性增高，超氧阴离子阳性细胞数减少，外毛细胞的cleaved caspase-3阳性细胞数降低。结论：Vitamin E能减轻与年龄有关的听力丧失，这种作用可能与抑制耳蜗氧化应激、外毛细胞丧失、静纤毛结构改变有关。
关键词：
维生素E；CBA小鼠；年龄相关性听力损失
Protective effect and mechanism of vitamin E on age related hearing loss in CBA mice
CorrespondingAuthor: LI Xingcheng Email: fcclixc@zzu.edu.cn
DOI: 10.3978/j.issn.2095-6959.2019.11.003
Abstract
Objective: To examine the effect on Vitamin E on age related hearing loss in CBA mice and its mechanism. Methods: CBA mice were treated with food contained Vitamin E as a Vitamin E intervention group and standard food as a control group. Hair cells were calculated by F-actin with Alexa Fluor-488 labeled phalloidin. Stereocilia morphology were evaluated by Scan electron microscope. The levels of superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities were measured. The level of superoxide anion in outer hair cells by using a dihydroethidium probe. The number of cleaved caspase-3 positive outer hair cells was detected by immunofluorescence staining. Results: CBA mice aged 10 months had significant hearing loss in the control group. Compared with the control group, the auditory brainstem response (ABR) threshold was decreased, the number of outer hair cells was increased, the alteration of stereocilia was reduced, the levels of SOD and GPx activities in cochlea were increased, the number of superoxide anion-positive cells in outer hair cells was decreased, the number of cleaved caspase-3 positive cells in outer hair cells was decreased in mice from the Vitamin E intervention group. Conclusion: Vitamin E can relieve age-related hearing loss, which may be related to the inhibition of cochlear oxidative stress, loss of outer hair cells, and structural changes of stereocilia.
Keywords：
vitamin E; CBA mice; age related hear loss