Objective: To Explore effect of PAR-2 splice variant of PXR (PXR2) polymorphism on the rate of tacrolimus blood concentration and drug dose (C/D) in renal transplant patients. Methods: We collected the 73 blood preparations of the renal transplant patients in the Organ Transplant Department of Jiangxi Province People’s Hospital from July 2014 To June 2017. Detected the PXR2 polymorphism by the means of Anelic Special-Touch Down PCR. Recorded the age, sexual, weight, hemoglobin value, total bilirubin value and the use of proton-pump inhibitor. Results: The PCR show that there are three genes types which include 39 homozygote type (53.42%), 4 wild type (5.48%) and 30 heterozygote type (41.1%). The multiple linear regression shows that the 6 bp-deletion polymorphism in the promotor region of PXR2 is unrelated to C/D, and the difference was not statistically significant (P>0.05). The hemoglobin value and total bilirubin value was changing positively with the tacrolimus C/D and the difference was statistically significant (P<0.05). The One-Way ANOVA indicated that there was no statistically significant difference of C/D values between the three groups of homozygous, wild and heterozygous types on 3 days, 2 weeks and 1 month after the operation. Conclusion: The 6 bp-deletion polymorphism in the promotor region of PXR2 had nothing to do with Tacrolimus C/D. The hemoglobin value and total bilirubin value was changing positively with the Tacrolimus C/D.