抗生素相关性腹泻的代谢组学
| 作者: |
1李东东,
1霍巧玲,
1刘宝清
1 北京中医药大学东方医院普外科，北京 100078 |
| 通讯: |
刘宝清
Email: Liubaoqing315@sina.com |
| DOI: | 10.3978/j.issn.2095-6959.2019.07.003 |
摘要
目的：从代谢水平上探究抗生素相关性腹泻(antibiotic associated diarrhea，AAD)。方法：将SD大鼠分为空白对照组、AAD组进行造模，观察一般情况变化并运用超高液相色谱-四极杆飞行时间质谱检测大鼠血清；一般统计数据用SPSS 20.0分析，代谢组学数据采用Markerlynx软件分析。 结果：造模第10天，与空白对照组相比，AAD组鉴定出22个生物学标志物，其中与AAD相关的代谢通路中影响因子较高的通路是缬氨酸，亮氨酸和异亮氨酸降解。结论：从代谢组学上认为AAD与氨基酸代谢密切相关。
关键词：
代谢组学；抗生素相关性腹泻；支链氨基酸降解
Metabonomics on antibiotic associated diarrhea
CorrespondingAuthor: LIU Baoqing Email: Liubaoqing315@sina.com
DOI: 10.3978/j.issn.2095-6959.2019.07.003
Abstract
Objective: To explore the antibiotic associated diarrhea (AAD) from metabolic level. Methods: SD rats were divided into a blank control group and an AAD group to establish models, observe the general changes and detect rat serum by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry; general statistical data were analyzed by SPSS 20.0, and metabolomics data were analyzed by Markerlynx software. Results: On the 10th day of modeling, compared to the blank control group, twenty-two identical endogenous biomarkers were screened out in the AAD group. Among the metabolic pathways related to AAD, the ones with higher influencing factors are: valine, leucine and isoleucine degradation. Conclusion: Metabolomics suggests that AAD is closely related to amino acid metabolism.
Keywords：
metabonomics; antibiotic associated diarrhea; BCAAS degradation