文章摘要

HIP1基因沉默对人食管癌EC109 细胞迁移侵袭能力的影响

作者: 1冯征, 1王雪娇, 1文苗苗, 1夏靖华, 1张晏宁, 1张娇, 1张志培, 1孙盈
1 空军军医大学第二附属医院胸外科，西安 710038
通讯: 张志培 Email: zzpzyy@fmmu.edu.cn
孙盈 Email: suny_an1314@163.com
DOI: 10.3978/j.issn.2095-6959.2019.06.001
基金: 空军军医大学唐都医院创新发展基金 (2016JCYJ009)。

摘要

目的：研究亨廷顿蛋白相互作用蛋白1(Huntingtin interacting protein 1，HIP1)基因沉默对人食管癌EC109细胞迁移侵袭的影响。方法：构建慢病毒干扰HIP1的食管癌细胞系，定量PCR和蛋白质印迹法检测HIP1基因沉默效果。通过细胞划痕实验和Transwell迁移侵袭试验研究HIP1基因沉默对人食管癌细胞迁移侵袭能力的影响。结果：慢病毒转染EC109食管癌细胞后，HIP1基因沉默效率达到80%以上。qRT-PCR和蛋白质印迹法证实此次慢病毒干扰HIP1效果显著。细胞划痕试验和Transwell迁移侵袭试验显示HIP1基因沉默后可抑制EC109食管癌细胞的迁移和侵袭。结论：构建的HIP1干涉慢病毒表达载体可抑制HIP1基因和蛋白的表达，并可有效抑制EC109食管癌细胞迁移和侵袭，提示HIP1作为食管癌的差异蛋白，可能通过促进食管癌细胞的转移参与食管癌的发生发展。
关键词： 亨廷顿蛋白相互作用蛋白1；慢病毒干扰；迁移与侵袭；食管癌

Effect of HIP1 gene silencing on migration and invasion of EC109 cells in human esophagus cancer

Authors: 1FENG Zheng, 1WANG Xuejiao, 1WEN Miaomiao, 1XIA Jinghua, 1ZHANG Yanning, 1ZHANG Jiao, 1ZHANG Zhipei, 1SUN Ying
1 Department of Thoracic Surgery, Second Affliated Hospital, Air Force Medical University, Xi’an 710038, China

CorrespondingAuthor: ZHANG Zhipei Email: zzpzyy@fmmu.edu.cn

DOI: 10.3978/j.issn.2095-6959.2019.06.001

Foundation: This work was supported by Air Force Medical University Tangdu Hospital Innovation and development Foundation, China (2016JCYJ009).

Abstract

Objective: To investigate the effect of Huntingtin-interacting protein 1 (HIP1) gene silencing on the migration and invasion of human esophagus cancer EC109 cells. Methods: Lentivirus interference of target HIP1 was structured and used to transfect into EC109 cells. Quantitative PCR (qPCR) and Western blotting were adopted to detect the HIP1 gene silencing effect. Moreover, the wound healing assay and transwell migration and invasion were performed to analyze the effect of HIP1 on the migration and invasion of EC109 cells. Results: The efficiency of HIP1 gene silencing was about 80% after EC109 cell transfection. qPCR and Western blot proved the significant effect of lentivirus interference. The wound healing, transwell migration and invasion assay showed that the migration and invasion of EC109 cells were inhibited by shRNA-HIP1. Conclusions: Lentivirus interference of target HIP1 can specifically suppress the gene and protein expression of HIP1. In addition, HIP1 gene silencing can inhibit the migration and invasion of EC109 cells. Therefore, we suggest that HIP1 may affect the prognosis of esophageal cancer by promoting the metastasis.
Keywords： Huntingtin interacting protein 1; lentivirus interference; migration and invasion; esophagus cancer

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