Objective: To investigate the effect of sequential enteral nutrition (EN) on hemorrhagic stroke with swallowing dysfunction and its effects on neurological function, immune function, intestinal mucosal barrier and prognosis. Methods: A total of 145 patients with hemorrhagic stroke and swallowing dysfunction admitted to our hospital from October 2016 to April 2018 were divided into experimental group (75 cases) and control group according to the selected enteral nutrition. For example, the control group was treated with conventional EN, and the experimental group was treated with sequential EN for 15 days. The NIHSS score, intestinal mucosal barrier index [endotoxin (ET), diamine oxidase (DAO)], and nutritional index [albumin (ALB), prealbumin (PAB), transferrin (TRF)], immune markers (IgM, IgA, IgG, CD3⁺, CD4⁺, CD4⁺/CD8⁺), complications (pulmonary infection, stress ulcer, deep vein thrombosis) and 28-d fatality rate were compared between the two groups. Results: There was no significant difference in NIHSS score between the two groups (P>0.05). The levels of ET and DAO in the experimental group were lower than those in the control group, the difference was significant (P<0.05). In terms of nutritional indicators, the ALB, PAB and TRF levels in the experimental group. The level of IgM, IgA, IgG, CD3⁺, CD4⁺, CD4⁺/CD8⁺ in the experimental group was higher than that in the control group (P<0.05). The incidence of complications in the experimental group was lower than that in the control group (P<0.05). There was no significant difference in the 28-day mortality between the two groups (P>0.05). Conclusion: Sequential EN can improve the nutritional index of patients with hemorrhagic stroke with swallowing dysfunction, improve immune function, improve intestinal mucosal barrier and reduce complications. However, it has no significant improvement on neurological function and prognosis.