Objective: To evaluate the effects of different educational methods on the use of the electronic pen. Methods: In this study included 100 children with growth hormone deficiency, we used different methods (self-study and physician guidance) to learn how to use the electronic pen, evaluate the effect of educational methods on the use of electronic pen for injection of recombinant human growth hormone injection, and summarize the common problems in the use of electronic pen. Results: Compared with their own learning, guidance of physician could reduce parents use the electronic pen error rates. In the two groups using the increases the main problem in the process of the electronic pen to rotate the needle too much effort, at the injection site in umbilical week 5 centimeters of above, no exhaust operation and no at the injection site by inside and rubbing alcohol cotton piece wiped. Self-study is prone to install oblique inserting needles direction, not vertical insert, no bottle disinfection, after the injection time is not enough, not until the prompt the needle, didn’t check the refrigerator temperature, and close to the wall storage refrigerator drug problem. Conclusion: Professional guidance is helpful to improve parents’ ability to use the electronic pen and reduce the error rate.