Objective: To explore the diagnostic value of Tp-Tec, QTc and Tp-Te/QT ratio in acute ventricular arrhythmia in patients with AMI. Methods: The electrocardiogram of 186 patients with acute myocardial infarction admitted to our hospital from October 2014 to October 2017 was retrospectively analyzed. According to whether ventricular tachycardia and/or ventricular fibrillation occur in the acute stage, the patients were divided into the ventricular arrhythmia group (observation group, n=106) and the non-ventricular arrhythmia group (control group, n=80). The first electrocardiogram (the acute stage) of all patients within 24 h of the course of disease and the electrocardiogram at discharge(the recovery stage) were measured. The Tp-Tec, QTc and the Tp-Te/QT ratio of the enrolled population were analyzed. Results: The Tp-Tec, QTc and Tp-Te/QT ratio in the recovery stage of the two groups were significantly lower than those in the acute stage, and the differences were statistically significant (P<0.05). Compared with the control group, the Tp-Tec, QTc and Tp-Te/QT ratio of patients in the observation group in the acute stage were significantly increased, and the differences were statistically significant (P<0.05), while the differences in electrocardiogram indexes between the two groups in the recovery stage were not significant (P>0.05). The correlation analysis showed that Tp-Tec, QTc and Tp-Te/QT ratio were positively correlated with the occurrence of acute ventricular arrhythmia (r=0.761, P=0.015; r=0.823, P=0.039; r=0.156, P<0.001, respectively). The predictive value of Tp-Tec, QTc and Tp-Te/QT ratio on ventricular arrhythmias in patients with AMI were analyzed by ROC curve. The results showed that the Tp-Tec was the predictive index of acute ventricular arrhythmia with the optimal critical value of 125.26 ms and the area under the ROC curve of 0.9218, which had high diagnostic value; the QTc as the prediction index, the optimal critical value was 433.32 ms, the area under the ROC curve was 0.8231, and the diagnostic value was moderate. The diagnostic value is medium; the Tp-Te/QT ratio as the prediction index, the optimal critical value was 0.29, and the area under the ROC curve was 0.9608, with had the highest diagnostic value. Conclusion: Tp-Tec, QTc, Tp-Te/QT ratio were significantly positively correlated with the occurrence of acute ventricular arrhythmia in AMI patients, which had predictive value for the occurrence of ventricular arrhythmia. Meanwhile, Tp-Tec, QTc, Tp-Te /QT ratio were helpful for the evaluation of the prognosis of AMI patients.