SETDB1在胃癌中的表达及其临床意义
| 作者: |
1李金梅,
1陈红,
2杜强,
1王旭,
1马秋双,
3李小莉,
1张金库
1 保定市第一中心医院病理科，河北 保定 071000 2 新疆生产建设兵团第二师库尔勒医院病理科，新疆 库尔勒 841000 3 临沂市人民医院病理科，山东 临沂 276000 |
| 通讯: |
李小莉
Email: lixiaolihu@163.com 张金库 Email: 2286917939@qq.com |
| DOI: | 10.3978/j.issn.2095-6959.2019.04.006 |
| 基金: | 保定市科技局计划内项目 (17ZF220)；河北省重点研发计划健康医疗与生物医药专项项目 (18277732D)。 |
摘要
目的：探讨SETDB1在胃癌组织中的不同亚细胞定位表达及临床意义。方法：用SP免疫组织化学法检测161例胃癌和161例癌旁正常胃组织标本中SETDB1的表达情况。结果：在胃癌组织中SETDB1阳性表达定位于细胞质和细胞质伴细胞核，77例在细胞质阳性表达，其中45例伴有细胞核表达；在癌旁正常胃组织中，SETDB1阳性表达定位于细胞质；SETDB1的细胞质表达与临床病理特征不相关(P>0.05)；SETDB1的细胞核表达与胃癌浸润深度、淋巴结转移和pTNM分期相关(P<0.05)，与其他病理特征无关。生存和预后分析结果显示：SETDB1细胞核阳性表达组患者术后生存时间低于SETDB1阴性表达组(P<0.05)，胃癌浸润深度、淋巴结转移、pTNM分期和SETDB1细胞核阳性表达均是影响胃癌患者预后的重要因素，但只有pTNM分期是胃癌患者预后的独立影响因素。 结论：胃癌细胞核中的SETDB1异常表达可能参与了胃癌的发生发展，其高表达可能和胃癌的恶性生物学行为及预后评估相关。
关键词：
胃癌；SETDB1；预后；亚细胞定位
Expression and significance of the SETDB1 in gastric cancer
CorrespondingAuthor: LI Xiaoli Email: lixiaolihu@163.com
DOI: 10.3978/j.issn.2095-6959.2019.04.006
Foundation: This work was supported by Projects Planned by Baoding Science and Technology Bureau (17ZF220) and Key R&D Program of Special Projects of Health Care and Biomedicine of Hebei Province (18277732D), China.
Abstract
Objective: To investigate the significance of SETDB1 protein located at different sites of cells in gastric cancer and adjacent normal tissues. Methods: SP immunohistochemical stain (IHC) was used to examine the expression of SETDB1 in 161 cases of gastric cancer and adjacent tissues. Results: The SETDB1 cytoplasmic positivity was observed in 77 cases, whereas 45 of them accompanied with nuclear expression. SETDB1 cytoplasmic positive expression was not correlated with clinicopathological features of gastric adenocarcinoma. But the SETDB1 nuclear positivity was correlated with the depth of tumor infiltration, pTNM staging, lymph node metastasis. Kaplan-Meier single factor and Log-rank analysis showed that the SETDB1 nuclear positivity, lymph node metastasis, depth of cancer invasion and pTNM staging were all the important prognostic factors for patients with gastric cancer, the survival time of patients with SETDB1 nuclear positivity was lower than that of SETDB1 nuclear negativity (P<0.05); COX regression model analysis showed that pTNM staging was important independent prognostic factors. Conclusion: The abnormal expression of SETDB1 in the cell nucleus may be involved in the development of gastric cancer, and its high expression may be related to the malignant biological behavior and poor prognosis of gastric cancer.
Keywords：
gastric cancer; SETDB1; subcellular localization; prognosis