Objective: To explore the adult granulosa cell tumor ascites cells of the pathological characteristics and diagnostic criteria. Methods: We analyzed 18 cases of ovarian granulosa cell tumor ascites liquid-based cytology in the diagnosis of adult type wax, quantitative analysis of DNA and cell block immunocytochemistry results, and reviewed the relevant literature. Results: AGCT ascites liquid-based cytology in the diagnosis of 13 cases of mild nuclear heterogeneous cells, small round cell tumor in 5 cases. Liquid based cells which are scattered in the round, small polygonal cells, part of the dough, nuclear membrane folds, nucleus in obviously ditch, chromatin loose bubble or density, less cytoplasm, the cell can be naked nucleus shape or melon seeds, occasional Call-Exner corpuscle. DNA quantitative analysis are all negative. Immunocytochemistry, revealed positivity for α-inhibin, S-100, Calretinin and CD99, and Ki-67 index ranges from 2% to 10%. Conclusion: Adult ovary granulosa cell tumor ascites combined liquid-based cytology and DNA double body chemical analysis and immune cell can accurate conclusion, is helpful to clinical diagnosis and treatment.