MiR-377 靶向调控 Egr1 在乙肝肝纤维化病理机制中的作用
| 作者: |
1李贻弘,
1张宏,
1侯波,
1熊首先
1 江汉油田总医院消化科，湖北 潜江 433124 |
| 通讯: |
熊首先
Email: 616587532@qq.com |
| DOI: | 10.3978/j.issn.2095-6959.2019.03.004 |
摘要
目的：研究miR-377靶向调控早期生长反应因子(early growth response l，Egr1)在肝纤维化病理机制中的作用。方法：RT-qPCR检测肝纤维化患者血清中miR-377的表达；用miR靶基因数据库进行筛选，选择Egr1为miR-377的潜在靶基因；RT-qPCR和Western印迹法分别检测miR-377 mimics对Egr1的mRNA及蛋白表达的影响；CCK-8检测AngII诱导后HSC细胞和转染miR-377的HSC细胞增殖程度；RT-qPCR检测经AngII和AngII+miR-377刺激后与α-平滑肌动蛋白(α-smooth muscle actin， α-SMA)，FSP1和Collagen I的表达量。结果：MiR-377可能与肝纤维化的发生及发展有关；miR-377可以通过结合Egr1的3'-UTR直接抑制Egr1的mRNA翻译及其蛋白表达；miR-377可抑制肝细胞经AngII诱导后的增殖活性；miR-377能抑制经AngII刺激后α-SMA，FSP1和Collagen I的表达，在肝纤维化的进程中发挥抑制作用。结论：MiR-377通过抑制Egr1的表达抑制肝细胞的增殖和病理进程。
关键词：
肝纤维化；miR-377；早期生长反应因子；AngII
Role of miR-377 targeted regulation of EGR1 in the pathological mechanism of liver fibrosis in hepatitis B patient
CorrespondingAuthor: XIONG Shouxian Email: 616587532@qq.com
DOI: 10.3978/j.issn.2095-6959.2019.03.004
Abstract
Objective: To study the role of miR-377 targeted regulation of EGR1 in the pathological mechanism of liver fibrosis. Methods: The expression of miR-377 in serum of patients with liver fibrosis were detected by RT-qPCR. Egr1 was selected as the potential target gene of miR-377 by screening with miR target gene database. The effect of miR-377mimics on the mRNA and protein expression of Egr1 was detected by RT-qPCR and Western blot, respectively. CCK-8 was used to detect the proliferation of HSC cells and HSC cells transfected with miR-377 which were induced by AngII. RT-qPCR was used to detect the expression of α-smooth muscle actin (α-SMA), FSP1 and Collagen I after AngII and AngII + miR-377 stimulated. Results: MiR-377 may be related to the occurrence and development of liver fibrosis. miR-377 can directly inhibit the mRNA translation and protein expression of Egr1 by binding 3'-URT of Egr1. miR-377 could inhibit the proliferation of liver cells induced by AngII. miR-377 could inhibit the expression of α-SMA, FSP1 and Collagen I after AngII stimulated and miR-377 played an inhibitory role in the process of liver fibrosis. Conclusion: MiR-377 inhibits the proliferation and pathological process of liver cells by inhibiting the expression of Egr1.
Keywords：
hepatic fibrosis; miR-377; early growth response; AngII