Objective: To compare the sample satisfaction rate of 22G and 25G needles used in the ultrasound-guided fine needle aspiration cytology (US-FNAC) of thyroid nodules. Methods: A total of 166 patients, 166 thyroid nodules from January 2017 to January 2018 in our hospital were included in this study, each nodule was aspirated with both 22G and 25G needles guided by ultrasound. According to the Adler stratifications of vascularity, 166 thyroid nodules were divided into a non-hypervascular group (Adler level 0–2) and a hypervascular group (Adler level 3). All specimens were directly smeared on glass slides and subjected to Thinprep Cytologic Test. Samples were divided into satisfied or unsatisfied. The order of 22G and 25G needle was in terms of random table, then the satisfaction rate between 22G and 25G was compared. Results: The satisfaction rate of 22G needle aspiration of thyroid nodules was 83.1% (138/166), while the satisfaction rate of 25G needle aspiration of thyroid nodules was 89.8% (149/166). There was no statistically significant difference between 22G and 25G needles in terms of the sample satisfaction rate (P=0.07). There was no statistically significant difference in a non-hypervascular group either (80.4% vs 82.1%, P=0.81). However, the sample satisfaction rate of a hypervascular group showed significant difference (84.5% vs 93.6%, P=0.03). Conclusion: 25G needles can aspirate adequate material for cytopathological diagnosis, it may have a higher satisfaction rate in nodules with rich blood supply.