β-catenin在慢性移植肾肾损伤中的作用
| 作者: |
1杨迷玲,
1徐宪伟,
1张果,
2曲青山,
1杨金花
1 郑州人民医院病理科，郑州 450003 2 郑州人民医院器官移植科，郑州 450003 |
| 通讯: |
杨金花
Email: 13837108837@163.com |
| DOI: | 10.3978/j.issn.2095-6959.2019.02.006 |
| 基金: | 郑州市科技攻关计划项目(20150078)。 |
摘要
目的：探讨β-catenin在慢性移植肾肾损伤中的作用。方法：选取诊断为慢性移植肾肾损伤的肾活检标本30例和正常肾组织10例做对照；用免疫组织化学法检测肾活检组织中β-catenin，CD163(标记M2型巨噬细胞)和SMA蛋白的表达情况；用Masson染色法评估肾活检组织的间质纤维化程度；分析肾活检组织中β- catenin与CD163、移植肾间质纤维化程度及患者血肌酐(Cr)水平的关系。 结果：移植肾组织中的β-catenin、CD163和SMA显著高于正常肾组织(P<0.05)；随着移植肾间质纤维化程度的加重，移植肾组织中β-catenin和CD163的表达逐渐增多，患者血肌酐和尿素氮水平也逐渐升高(P<0.05)；移植肾组织中β-catenin和CD163的表达呈正相关，与患者血肌酐水平呈正相关(P<0.001)。结论：β-catenin和CD163阳性的M2型巨噬细胞在慢性移植肾肾损伤组织中异常高表达，并且与移植肾间质纤维化程度和肾移植患者肾功能不全相关。
关键词：
肾移植；β-catenin；M2型巨噬细胞；间质纤维化
Role of β-catenin in chronic kidney allograft injury
CorrespondingAuthor: YANG Jinhua Email: 13837108837@163.com
DOI: 10.3978/j.issn.2095-6959.2019.02.006
Foundation: This work was supported by Zhengzhou Science and Technology Tackling Key Project Foundation, China (20150078).
Abstract
Objective: To investigate the role of β-catenin in chronic kidney allograft injury. Methods: By using immunohistochemical staining method, the levels of β-catenin, CD163 and SMA expression were detected in the renal biopsy tissues of 30 cases of CAI and 10 cases of normal kidney. The degree of interstitial fibrosis in renal biopsy tissues was assessed by Masson staining. The relationship between β-catenin and CD163, the degree of interstitial fibrosis and serum creatinine level in patients was analysed. Results: There were statistically significant differences in expression of β-catenin, CD163 and SMA between kidney allograft and normal kidney tissue (P<0.05); with the increase of renal fibrosis severity, the levels of serum Cr, BUN and the expression of β-catenin and CD163 were increased (P<0.05); the β-catenin protein was positively correlated with the expression of CD163 and serum creatinine (P<0.001). Conclusion: The abnormal high expression of β-catenin and CD163 positive (M2 macrophages) in chronic kidney allograft injury are associated with the degree of interstitial fibrosis and renal insufficiency in patients.
Keywords：
kidney transplant; β-catenin; M2-type macrophages; interstitial fibrosis