Objective: To investigate the relationship between sevoflurane and invasion, migration of breast cancer MDA-MB-231 cells and its mechanism. Methods: MDA-MB-231 cells were treated with different concentrations of sevoflurane for 6 h. The experiment was divided into a control group, a 1.7% administration group, a 3.4% administration group, and a 5.1% administration group. The migration and invasion ability of cells was observed by scratch test and Transwell chamber assay. The protein levels of matrix metalloprotease-2 (MMP-2), MMP-9, E-cadherin and β-catenin were examined by Western blot. Results: Compared with the control group, the migration and invasion of MDA-MB-231 cells treated with sevoflurane significantly decreased (P<0.05). The levels of MMP-2, MMP-9, E-cadherin, and β-catenin in MDA-MB-231 cells treated with sevoflurane were significantly decreased with an increase of concentration (P<0.05). The LiCl, a Wnt/β-catenin signaling pathway activator, which interacted with 5.1% sevoflurane for 6 h, and the levels of β-catenin and E-cadherin were significantly increased, and the invasion and migration ability were significantly increased. Conclusion: Sevoflurane can inhibit the invasion and migration of breast cancer MDA-MB-231 cells in a concentration-dependent by regulating Wnt/β-catenin signaling pathway and MMP-2 and MMP-9 protein levels, providing a new theoretical basis for the treatment of breast cancer.