Objective: To investigate the clinical therapeutic efficacy of Yinqiao powder combined with amoxicillin on curative effect, pain and inflammatory factors in patients with acute tonsillitis. Methods: Ninety patients with acute tonsillitis treated in our hospital from January 2015 to September 2017 who were selected as the subjects. All the patients were divided into a study group (45 cases) and a control group (45 cases) according to the random digital table method. After symptomatic supportive therapy such as fever abatement, water and electrolyte disturbance correction and cefathiamidine anti-infection, the control group was used with amoxicillin treatment regimen, the study group was given Yinqiao powder on the basis of the treatment. The clinical efficacy, pain level, inflammatory factor level, and immune function of the 2 groups were compared. Results: After the combined treatment, the clinical efficiency of the study group was significantly higher than that of the control group (P<0.05). In the study group, the time of disappearance of the sore throat, fever subsided and tonsil reduction were remarkably shorter than those in the control group (P<0.01). Two groups of patients after different treatments for 7 days, the levels of inflammatory factors such as IL-6 and TNF-α which were remarkably lower than those before the treatment (P<0.01), and the indexes of the study group were decreased more remarkably than those in the control group (P<0.01). Compared with before treatment, two groups of patients after different treatments for 7 days the scores of VAS and FPS-R were remarkably reduced (P<0.01), and the indexes in the study group decreased more remarkably than those in the control group (P<0.01). Conclusion: The treatment of acute tonsillitis with Yinqiao powder combined with amoxicillin can significantly improve its clinical symptoms, reduce inflammation and relieve pain.