下调miRNA-181a降低寒冷刺激诱导的妊娠高血压小鼠的血压
| 作者: |
1王琳,
2史锡岩,
3杨建敏,
3薛国勇
1 南阳地质医院妇产科，河南 南阳 473000 2 南阳地质医院内科，河南 南阳 473000 3 南阳市中心医院妇产科，河南 南阳 473000 |
| 通讯: |
王琳
Email: wanglinmed@163.com |
| DOI: | 10.3978/j.issn.2095-6959.2018.11.002 |
摘要
目的：检测miRNA-181a在寒冷刺激诱导的妊娠高血压小鼠胎盘组织中的表达，研究下调miRNA-181a表达后对妊娠高血压小鼠血压的影响，并探讨初步机制。方法：尾静脉注射200 nmol/kg miRNA-181a-antagomir，2 d后，通过反复寒冷应激刺激方法建立妊娠高血压小鼠模型。颈动脉插管法测量小鼠血压，qPCR检测胎盘组织中miRNA-181a的表达，使用NO试剂盒测量血清中NO含量，ELISA检测血清中血红素氧合酶-1(heme oxygenase 1，HO-1)含量，Western印迹法检测胎盘组织中Akt，pAkt，内皮型一氧化氮合酶(eNOS)和peNOS蛋白表达。结果：与对照组相比，寒冷刺激诱导的妊娠高血压小鼠胎盘组织中miRNA-181a水平显著增加。与模型组相比，沉默miRNA-181a能够降低孕鼠的血压，升高血清NO和HO-1水平，并上调胎盘组织中pAkt和peNOS蛋白表达。结论：miRNA-181a表达可能与寒冷刺激诱导的妊娠高血压的发生发展有潜在关系。
关键词：
妊娠高血压；miRNA-181a；Akt；内皮型一氧化氮合酶
Down-regulation miRNA-181a reduces blood pressure in cold-induced pregnant hypertensive mice
CorrespondingAuthor: WANG Lin Email: wanglinmed@163.com
DOI: 10.3978/j.issn.2095-6959.2018.11.002
Abstract
Objective: To examine the expression of miRNA-181a in placenta tissue of cold induced-pregnant hypertensive mice and to explore the effects of and the possible mechanisms of down-regulation miRNA-181a on blood pressure. Methods: Mice were injected with 200 nmol/kg miRNA-181a-antagomir via tail vein. Two days later, model of pregnant hypertension was established by cold-stress stimulation method. Blood pressure was measured with direct intubation of carotid. The expressions of miRNA-181a in placenta tissues were examined by qPCR; the levels of serum NO were determined by NO assay kit; the levels of serum heme oxygenase 1 (HO-1) were determined by ELISA assay; The protein expressions of Akt, pAkt, eNOS and peNOS were analyzed by Western blot. Results: Compared to control group, the expression of miRNA-181a in placenta tissue of cold induced-pregnant hypertensive mice was significantly increased. Moreover, compared to model group, inhibition of miRNA-181a suppressed the blood pressure, increased the levels of serum NO and HO-1, and further, increased the protein expression of pAkt and peNOS in placenta tissue, respectively. Conclusion: MiRNA-181a may be related to the development of cold induced-pregnant hypertension.
Keywords：
pregnant hypertensive; miRNA-181a; Akt; eNOS