Objective: To study the status of pre-hospital delay in patients with acute myocardial infarction (AMI), and to analyze the related factors, focusing on analysis of influencing factors of patients. Methods: A total of 240 patients aged 18–89 years who were admitted to the Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical University from April 2014 to April 2016 were enrolled in the study. Then we collected and recorded the following information about the patient: the basic situation (age and sex), clinical data, semi-closed survey patients’ reasons for delay to seek medical help and reasons for choice of first medical contact. Results: In 240 cases, 32.92% of patients had pre-hospital delay, 12.08% of patients visited hospital 24 h after the onset of symptoms. Logistic regression analysis showed that atypical symptoms, not to attribute to true cause diabetes mellitus and not called emergency medical services (EMS) were risk factors for prehospital delay. Atypical symptoms and a history of diabetes were risk factors for incorrect attribution. Conclusion: In patients with AMI, misinterpretation of symptoms is common, and typical symptoms are tended to correct attribution of symptoms and reduce the risk of pre-hospital delay. EMS is the best way to transport patients with AMI, which can shorten the time before admission.