miR-128-3p 在宫颈癌中的表达及其发病机制
| 作者: |
1张华东,
1张显峰,
1刘桂琴,
1闫晓歌
1 平顶山市第五人民医院病理科，河南 平顶山 467000 |
| 通讯: |
张显峰
Email: 13837599281@163.com |
| DOI: | 10.3978/j.issn.2095-6959.2018.07.005 |
摘要
目的：研究miR-128-3p在宫颈癌(cervical carcinoma，CC)组织及癌旁组织中的表达差异，并进一步 探索其在CC发病中的机制。方法：收集于平顶山市第五人民医院就诊的CC患者癌组织标本，采 用荧光素酶报告基因检测HEK293细胞株中miR-128-3p与BMI1靶向关系，Western印迹法验证miR- 128-3p BMI1蛋白的表达水平，实时荧光定量PCR(real-time quantitation PCR，RT-qPCR)法检测 CC组织及癌旁组织中miR-128-3p及BMI1的相对表达含量，流式细胞术法检测miR-128-3p过表达后 HeLa细胞凋亡情况。结果：miR-128-3p靶向结合于BMI1的3'UTR区；miR-128-3p在CC中低表达， BMI1在CC中高表达；miR-128-3p过表达下调BMI1表达，并促进HeLa细胞凋亡。结论：miR-128- 3p在CC患者中低表达，可能通过靶向BMI1发挥其生物学效应，并促进HeLa细胞凋亡。
关键词：
miR-128-3p；宫颈癌；BMI1
Expression of miR-128-3p in cervical cancer and its pathogenesis
CorrespondingAuthor: ZHANG Xianfeng Email: 13837599281@163.com
DOI: 10.3978/j.issn.2095-6959.2018.07.005
Abstract
Objective: To investigate the differential expression of miR-128-3p in cervical carcinoma (CC) tissue and paracancerous tissue, and to explore its pathogenic role in CC. Methods: The specimens of CC patients were collected in the Fifth People’s Hospital of Pingdingshan. Luciferase reporter gene assay was used to explore targeting regulatory relations between miR-128-3p and BMI1 in HEK293. Western blot was employed to verify the expression of BMI1 protein, the potential target of miR-128-3p. Real-time quantitation PCR (RT-qPCR) was conducted to detect the relative expression of miR-128-3p and BMI1 in CC tissue and paracancerous tissue. Flow cytometry experiment was performed to detect apoptosis of HeLa cells after overexpression of miR-128-3p. Results: miR-128-3p targeted the 3'UTR region of BMI1. Low expression of miR-128-3p was found,while high expression of BMI1 found in CC. Overexpression of miR-128-3p down-regulated the expression of BMI1, and promoted the apoptosis of HeLa cells. Conclusion: MiR-128-3p is low expressed in CC patients and may bring biological effects by targeting BMI1. miR-128-3p promotes the apoptosis of HeLa cells.
Keywords：
miR-128-3p; cervical cancer; BMI1