MCM2 与P16 在子宫颈病变细胞蜡块与活检标本中的表达
| 作者: |
1左元元,
1徐洪明,
1黄建达,
1毛海峰,
1孙岳军
1 江阴市人民医院病理科，江苏 江阴 214400 |
| 通讯: |
孙岳军
Email: syj996633@126.com |
| DOI: | 10.3978/j.issn.2095-6959.2018.07.004 |
| 基金: | 江阴市科技局社会发展科技示范项目( JYKJ3308)。 |
摘要
目的：探讨微型染色体维持蛋白2(minichromosome maintenance protein 2，MCM2)及P16在子宫 颈病变细胞蜡块及组织学标本中的表达。方法：采用免疫组织化学染色EnVision法检测118例宫 颈液基细胞学细胞蜡块，126例宫颈活检组织中MCM2及P16的表达。结果：在无上皮内病变或 恶性病变(no intraepithelial or malignant lesions，NILM)，意义不明的非典型鳞状细胞(atypical squamous cells with unclear significance，ACS-US)，低度鳞状上皮内病变(low degree squamous intraepithelial lesion，LSIL)，非典型鳞状细胞，不能排除高度鳞状上皮内病变(atypical squamous cells, cannot exclude high-grade squamous intraepithelial lesion，ASC-H)及高度鳞状上皮内病变 (high degree squamous intraepithelial lesion，HSIL)的细胞蜡块样本中，MCM2的阳性率分别为 0%，22%，55%，86%和92%，P16的阳性率分别为0%，9%，27%，68%和100%，差异有统计学 意义(P<0.01)；在宫颈上皮内瘤变1级(cervical intraepithelial neoplasia grade 1，CIN1)，CIN2， CIN3和宫颈癌组织样本中，MCM2的阳性率分别为0%，50%，81%，97%和100%，P16阳性率 分别为0%，18%，77%，100%和100%，差异有统计学意义(P<0.01)；在人乳头瘤状病毒(human papillomavirus，HPV)16/18感染，其他高危型HPV感染以及无HPV感染的病例中，MCM2的阳 性率分别为86%，36%和8%，P16的阳性率分别为89%，14%和0%，差异有统计学意义(P<0.01)。 结论：MCM2和P16与宫颈病变程度高度相关，可用于非典型鳞状上皮细胞及低度鳞状上皮内病变 人群的分流管理。
关键词：
微型染色体维持蛋白2；P16；宫颈病变；细胞蜡块
Expression of MCM2 and P16 in cell block and biopsy samples of cervical lesion
CorrespondingAuthor: SUN Yuejun Email: syj996633@126.com
DOI: 10.3978/j.issn.2095-6959.2018.07.004
Foundation: This work was supported by the Jiangyin Municipal Transformation of Scientific and Technological Achievements Project, China ( JYKJ3308).
Abstract
Objective: To determine the expression of minichromosome maintenance protein 2 (MCM2) and P16 protein in cell block and tissue samples of cervical lesion. Methods: Immunohistochemical EnVision method was employed to investigate the expression of MCM2 and P16 in 118 cell blocks of liquid-based cytology of cervical screening and 126 cervical biopsy tissue samples. Results: Positive rates of immunostaining for MCM2 on no intraepithelial or malignant lesions (NILM), atypical squamous cells with unclear significance (ACS-US), low degree squamous intraepithelial lesion (LSIL), atypical squamous cells, cannot exclude high-grade squamous intraepithelial lesion (ASC-H), high degree squamous intraepithelial lesion (HSIL) in cell block samples were 0%, 22%, 55%, 86%, and 92%, while positive rates of P16 were 0%, 9%, 27%, 68%, and 100%, with significant difference (P<0.001). Positive rates of MCM2 in the normal cervical epithelia, cervical intraepithelial neoplasia grade 1 (CIN1) group, CIN2 group, CIN3 group and cervical carcinoma were 0%, 50%, 81%, 97%, and 100% prespectively; while positive rates of P16 were 0%, 18%, 77%, 100%, and 100%, with significant difference (P<0.001). Positive rate of MCM2 were 86%, 36% and 8% in human papillomavirus (HPV) 16/18 group, other high-risk HPV group and HPV negative group, while positive rates of P16 were 89%, 14%, and 0%, with significant difference (P<0.001). Conclusion: The expression of MCM2 and P16 is correlated with increased dysplasia and lesion severity and could triage effectively in patients with atypical squamous cells of undetermined signification (ASCUS) and low grade squamous intraepithelial lesion (LSIL).
Keywords：
minichromosome maintenance protein 2; P16; cervical lesion; cell block