文章摘要

乳腺癌预后相关基因标志物筛选

作者: 1饶茜, 2胡豪飞, 2张丹, 2贺喜, 2邵玲, 2谢妮
1 深圳大学医学部,广东 深圳 518035
2 深圳市第二人民医院转化研究院,深圳大学第一附属医院,广东 深圳 518035
通讯: 谢妮 Email: xn100@szu.edu.cn
DOI: 10.3978/j.issn.2095-6959.2018.05.007
基金: 广东省自然科学基金(2016A030313029,2017A030313668);深圳市三名工程(SZSM201612031);深圳市政府项目(JCYJ20170817171808368, JCYJ20170818085657917)。

摘要

目的:筛选与乳腺癌预后相关的差异表达基因,为乳腺癌的预后评估及潜在靶点研究提供参考。方法:对乳腺癌组织及配对癌旁组织的基因进行二代高通量测序,筛选差异表达基因,并对其功能及预后进行分析。结果:筛选非三阴性乳腺癌上调差异表达基因共795个,下调基因为1 008个。三阴性乳腺癌上调差异表达基因共1 067个,下调基因1 034个。富集的主要通路有细胞周期、系统性红斑狼疮、DNA复制、病毒性致癌、酒精性中毒相关通路。结论:EIF4G1,CENPF上调可能与乳腺癌预后呈相关性,是潜在的乳腺癌治疗靶向目标。在提高乳腺癌生存率的潜在预后指标方面,SEPT2可提供更好的预后。
关键词: 乳腺癌;高通量测序;预后;靶向标志物

Screening of related genetic markers for breast cancer prognosis

Authors: 1RAO Xi, 2HU Haofei, 2ZHANG Dan, 2HE Xi, 2SHAO Ling, 2XIE Ni
1 Health Science Center, Shenzhen University, Shenzhen Guangdong 518035, China
2 Institute of Translation Medicine, Shenzhen Second People’s Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen Guangdong 518035, China

CorrespondingAuthor: XIE Ni Email: xn100@szu.edu.cn

DOI: 10.3978/j.issn.2095-6959.2018.05.007

Foundation: This work was supported by the Natural Science Foundation of Guangdong (2016A030313029, 2017A030313668)

Abstract

Objective: To screen differentially expressed genes related to the prognosis of breast cancer and provide a reference for the prognosis evaluation and potential target research of breast cancer. Methods: This study focused on the second-generation high-throughput sequencing of genes from breast cancer tissues and paired adjacent tissues to screen for differentially expressed genes and to analyze their function and prognosis. Results: A total of 795 differentially expressed genes were up-regulated in non-triple negative breast cancers, and the number of down-regulated genes was 1 008. Triple negative breast cancers up-regulated 1 067 differentially expressed genes and downregulated 1034 genes. The main pathways for enrichment are cell cycle, systemic lupus erythematosus, DNA replication, viral carcinogenesis, and alcohol-poisoning pathways. Conclusion: Up-regulation of EIF4G1 and CENPF may be associated with prognosis of breast cancer and are potential targets for breast cancer treatment. SEPT2 can provide a better prognosis in improving the prognosis of breast cancer.
Keywords: breast cancer; high-throughput sequencing; prognosis; targeted markers

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