乳腺癌预后相关基因标志物筛选
| 作者: |
1饶茜,
2胡豪飞,
2张丹,
2贺喜,
2邵玲,
2谢妮
1 深圳大学医学部，广东 深圳 518035 2 深圳市第二人民医院转化研究院，深圳大学第一附属医院，广东 深圳 518035 |
| 通讯: |
谢妮
Email: xn100@szu.edu.cn |
| DOI: | 10.3978/j.issn.2095-6959.2018.05.007 |
| 基金: | 广东省自然科学基金(2016A030313029，2017A030313668)；深圳市三名工程(SZSM201612031)；深圳市政府项目(JCYJ20170817171808368, JCYJ20170818085657917)。 |
摘要
目的：筛选与乳腺癌预后相关的差异表达基因，为乳腺癌的预后评估及潜在靶点研究提供参考。方法：对乳腺癌组织及配对癌旁组织的基因进行二代高通量测序，筛选差异表达基因，并对其功能及预后进行分析。结果：筛选非三阴性乳腺癌上调差异表达基因共795个，下调基因为1 008个。三阴性乳腺癌上调差异表达基因共1 067个，下调基因1 034个。富集的主要通路有细胞周期、系统性红斑狼疮、DNA复制、病毒性致癌、酒精性中毒相关通路。结论：EIF4G1，CENPF上调可能与乳腺癌预后呈相关性，是潜在的乳腺癌治疗靶向目标。在提高乳腺癌生存率的潜在预后指标方面，SEPT2可提供更好的预后。
关键词：
乳腺癌；高通量测序；预后；靶向标志物
Screening of related genetic markers for breast cancer prognosis
CorrespondingAuthor: XIE Ni Email: xn100@szu.edu.cn
DOI: 10.3978/j.issn.2095-6959.2018.05.007
Foundation: This work was supported by the Natural Science Foundation of Guangdong (2016A030313029, 2017A030313668)
Abstract
Objective: To screen differentially expressed genes related to the prognosis of breast cancer and provide a reference for the prognosis evaluation and potential target research of breast cancer. Methods: This study focused on the second-generation high-throughput sequencing of genes from breast cancer tissues and paired adjacent tissues to screen for differentially expressed genes and to analyze their function and prognosis. Results: A total of 795 differentially expressed genes were up-regulated in non-triple negative breast cancers, and the number of down-regulated genes was 1 008. Triple negative breast cancers up-regulated 1 067 differentially expressed genes and downregulated 1034 genes. The main pathways for enrichment are cell cycle, systemic lupus erythematosus, DNA replication, viral carcinogenesis, and alcohol-poisoning pathways. Conclusion: Up-regulation of EIF4G1 and CENPF may be associated with prognosis of breast cancer and are potential targets for breast cancer treatment. SEPT2 can provide a better prognosis in improving the prognosis of breast cancer.
Keywords：
breast cancer; high-throughput sequencing; prognosis; targeted markers