淋巴增强因子1及细胞周期蛋白D1在肝门部胆管癌中的表达及其临床意义
| 作者: |
1田立斌,
2张海光,
3鲁鑫,
3刘春玲
1 唐山市人民医院病理科，河北 唐山 063001 2 唐山市人民医院肝胆外科，河北 唐山 063001 3 唐山市人民医院 病理科，河北 唐山 063001 |
| 通讯: |
刘春玲
Email: lcl.1983.hi@163.com |
| DOI: | 10.3978/j.issn.2095-6959.2018.05.006 |
| 基金: | 河北省2017年度医学科学研究重点课题计划(20171275)。 |
摘要
目的：研究淋巴增强因子1(lymphoid enhancer factor-1，LEF-1)及细胞周期蛋白D1(cyclin D1，CCND1)在肝门部胆管癌(hilar cholangiocarcinoma，HC)中的表达及其临床意义。方法：应用免疫组织化学方法检测43例HC及对应癌旁正常组织中LEF-1及CCND1的表达，分析两者表达情况及其与临床病理参数的相关性。结果：LEF1在HC组织中的吸光度(OD)值为9 156±1 014，明显高于正常组织(2 132±906；P<0.05)；LEF1的表达与HC的肿瘤分化程度、TNM分期、淋巴结转移有关(均P<0.05)；CCND1在胆管癌组织中的OD值为11 204±9 075，明显高于正常组织(7 012±2 743；P<0.05)；CCND1的表达与HC的肿瘤分化程度、TNM分期、淋巴结转移有关(均P<0.05)；Spearman相关分析显示LEF-1与CCND1蛋白表达呈正相关(r=0.338，P=0.005)。结论：LEF-1和CCND1在HC组织中高表达，LEF-1/CCND1信号通路可能在HC的发生发展中发挥重要作用。
关键词：
肝门部胆管癌；淋巴增强因子1；细胞周期蛋白D1；临床病理参数
Expression and clinical significance of lymphoid enhancer factor-1 and cyclin D1 in patients with hilar cholangiocarcinoma
CorrespondingAuthor: LIU Chunling Email: lcl.1983.hi@163.com
DOI: 10.3978/j.issn.2095-6959.2018.05.006
Foundation: This work was supported by the Hebei Provincial Medical Science Research Key Project Foundation, China (20171275).
Abstract
Objective: To investigate the expression and clinical significance of lymphoid enhancer factor-1 (LEF-1) and cyclin D1 (CCND1) in patients with hilar cholangiocarcinoma (HC). Methods: Immunohistochemistry was used to analyze the expressions of LEF-1 and CCND1 in 43 cases of HC specimens and matched paratumorous normal specimens. The correlation of LEF-1 and CCND1 expression with clinicopathological variables of HC was carried out. Results: The OD value of LEF-1 expression in tumor tissue was 9 156±1 014; which was higher than that in adjacent tissue (2 132±906, P<0.05). The OD value of CCND1 in tumor tissue was higher than that in adjacent tissue (11 204±9 075 vs 7 012±2 743, P<0.05). LEF-1 or CCND1 expression was significantly associated with lymph node metastasis, histological grade, and TNM stage, respectively (all P<0.05). Correlation test showed that LEF1 and CCND1 were positively correlated in HC (r=0.338, P=0.005). Conclusion: The expressions of LEF-1 and CCND1 are higher in HC than those in the matched paratumorous normal specimens, which suggests that LEF1/CCND1 pathway may play an important role in the development of HC.
Keywords：
hilar cholangiocarcinoma; lymphoid enhancer factor-1; cyclin D1; clinicopathological parameter