Wnt11与ROCK2在食管鳞状细胞癌中的表达及其临床意义
| 作者: |
1苗娜,
1李俊芝,
2王志强,
3刘涛
1 新疆医科大学第一附属医院病理科，乌鲁木齐 830054 2 新疆武警总队医院神经内科，乌鲁木齐 830091 3 新疆医科大学第一附属医院临床医学研究院，乌鲁木齐 830054 |
| 通讯: |
刘涛
Email: lt02-16@163.com |
| DOI: | 10.3978/j.issn.2095-6959.2018.04.007 |
| 基金: | 省部共建国家重点实验室培育基地-新疆重大疾病医学重点实验室(SKL-HIDCA-2017-Y9)；新疆维吾尔自治区自然科学基金(2017D01C315)。 |
摘要
目的：研究Wnt/PCP通路中Wnt1l和ROCK2在食管鳞状细胞癌(esophageal squamous cell carcinoma，ESCC)组织中的表达及其与临床病理特征的关系。方法：采用免疫组织化学染色法，在250例石蜡包埋标本中检测Wnt11和ROCK2蛋白的表达变化。采用Western印迹在20例ESCC患者肿瘤组织和癌旁正常组织中检测Wnt11和ROCK2的表达情况。结果：Wnt11在正常食管组织中的阳性表达率为29.8%，而在ESCC组织中为31.9%，差异无统计学意义(P>0.05)；Rcok2在正常食管组织中的阳性表达率为12.3%，而在ESCC中的阳性表达率为56.5%，差异有统计学意义(P<0.05)。ROCK2在肿瘤组织的表达与其浸润血管相关(P<0.05)，而与肿瘤发生的部位、肿瘤分化程度、肿瘤T分期、肿瘤是否有淋巴结转移无关。在食管癌组织中Wnt11的蛋白表达与ROCK2的表达无明显相关性。
结论：在食管癌组织中，ROCK2的异常表达可能与食管癌的发生发展密切相关，其具体作用机制有待进一步深入研究。
关键词：
食管鳞状细胞癌；Wnt/PCP通路；Wnt11；ROCK2；肿瘤侵袭
结论：在食管癌组织中，ROCK2的异常表达可能与食管癌的发生发展密切相关，其具体作用机制有待进一步深入研究。
Expression of Wnt11 and ROCK2 in esophageal squamous cell carcinoma and its and clinical significance
CorrespondingAuthor: LIU Tao Email: lt02-16@163.com
DOI: 10.3978/j.issn.2095-6959.2018.04.007
Foundation: This work was supported by the Provincial Department of National Key Laboratory Cultivation Project-Xinjiang Key Laboratory of Major Diseases (SKL-HIDCA-2017-Y9) and the Xinjiang Uygur Autonomous Region Natural Science Foundation (2017D01C315), China.
Abstract
Objective: To investigate the expression and relationship of Wnt11, ROCK2 and clinical characteristics in esophageal squamous cell carcinoma (ESCC). Methods: Expression of Wnt11 and ROCK2 protein were examined on immunohistochemistry containing 260 paramn-embeded specimens of ESCC and specimens of normal cervical tissues. Expression of Wnt11 and ROCK2 protein were verified on Western blot containing 20 specimens of ESCC and specimens of normal cervical tissues. Results: The positive rates of Wnt11 protein in normal normal esophageal mucosa tissues were 29.8% and in esophageal carcinomas tissues were 31.9%, there was no significant difference between the two groups (P>0.05). The positive rates of ROCK2 protein in normal esophageal mucosa tissues were 12.3% and in esophageal carcinomas tissues were 56.5%, there was a significant difference between the two groups (P<0.05). The expression of ROCK2 protein was significantly related with the invation of vascular and there was no significantly difference between the expression of ROCK2 protein and ESCC patients’ tumour location, differentiation, T stage and lymph node metastisis. Conclusion: The abnormal expression of ROCK2 protein may promote the invasion of tumor cell.
Keywords：
esophageal squamous cell carcinoma; Wnt/PCP pathway; Wnt11; ROCK2; tumor invasion