Objective: To explore the effect of the cultivation of specialist breast nurses (SBN) under the mode of the multidisciplinary team (MDT), cultivation and application effect. Methods: We chose breast center 144 cases of hospitalized patients who were involved in the MDT team of breast cancer and 62 nurses as object of study, there were randomly divided into control group and experimental group, 72 cases of patients in each group, each group of 31 nurses. The experimental group of nurses were also involved in the MDT team in addition to the regular specialist training. The control group used routine specialist training. After 12 months of implementation, the doctors’ satisfaction and nurses’ knowledge of the nurses were compared. Before and after the MDT discussion comparison of the two groups of patients with uncertainty of the disease score and satisfaction with the nurse, nurses were involved in breast cancer MDT team 12 months. Results: After 12 months, doctors’ satisfaction and mammary gland specialized subject knowledge score of experimental group the nurse were superior to control group, the difference was statistically significant (P<0.05); after the MDT discussion, the experimental group patients, satisfaction and disease uncertainty were better than the control group, and the difference was statistically significant (P<0.05). Conclusion: Breast specialist nurses work throughout the MDT whole journey, the work for the development of MDT plays a good role, at the same time can increase patient and physician satisfaction, reduce the patients’ sense of disease uncertainty, increase the nurse mammary gland specialized subject knowledge, managers should attach importance to breast specialist nurses participate in MDT ability training and provide support.