Objective: To investigate the epidemiological characteristics of isolates of Streptococcus pneumoniae in Jingzhou area, and to provide evidence for clinical experience. Methods: A total of 3 415 cases of Streptococcus pneumoniae were detected in pediatric pediatric department (except neonates) from January 2015 to December 2016. A total of 342 strains of Streptococcus pneumoniae were detected, including 200 males, accounting for 58.48%; 142 females, accounting for 41.52%. The clinical data of Streptococcus pneumoniae and the resistance to various antibiotics were analyzed. Results: The detection rate of children with Streptococcus pneumoniae was not different from that of male children. Streptococcus pneumoniae mainly infected children under 5 years of age, seasonal distribution mainly in winter and spring; Streptococcus pneumoniae resistant to vancomycin and linezolid has not been detected; Streptococcus pneumoniae was more sensitive to levofloxacin, moxifloxacin, etanilapine, chloramphenicol, ofloxacin and telithromycin; and highly resistant to cotrimoxazole, tetracycline, erythromycin, penicillin; meropenem intermediates; amoxicillin, cefotaxime, ceftriaxone was still a high sensitivity. Conclusion: Amoxicillin, cefotaxime and ceftriaxone can be used as a clinical first-line drug for non-meningitis in Streptococcus pneumoniae infection in Jingzhou area. For high-resistant strains and severe infection, vancomycin and Linezolid.