SOX10在膀胱癌细胞中促进内皮细胞增殖、迁移及成管的作用
| 作者: |
1朱文
1 荆门市第二人民医院泌尿外科，湖北 荆门 448000 |
| 通讯: |
朱文
Email: 114528069@qq.com |
| DOI: | 10.3978/j.issn.2095-6959.2018.02.004 |
摘要
目的：探讨SOX10在膀胱癌细胞中促进内皮细胞增殖、迁移和成管中的作用。方法：利用Lipofectamine 2000在体外将SOX10 siRNA瞬时转染膀胱癌细胞株T24，转染48 h后利用Western印迹检测T24细胞系中SOX10表达；收集SOX10 siRNA转染的T24细胞的条件培养基，用MTT法、Transwell法和小管形成实验观察其对人脐静脉内皮细胞(human umbilical vein endothelial cell，HUVEC)的增殖、迁移和成管能力的影响。结果：T24细胞转染SOX10 siRNA后，SOX10蛋白表达明显降低。SOX10 siRNA转染组的条件培养基促HUVECs的增殖、迁移和成管能力较空白对照组和阴性对照组明显降低，差异有统计学意义(P<0.001)。结论：SOX10在膀胱癌中可通过旁分泌的方式影响内皮细胞的增殖、迁移和小管形成能力。
关键词：
SOX10；膀胱癌；内皮细胞；血管新生
Effect of SOX10 in bladder cancer cells on promoting endothelial cell proliferation, migration and tube formation
CorrespondingAuthor: ZHU Wen Email: 114528069@qq.com
DOI: 10.3978/j.issn.2095-6959.2018.02.004
Abstract
Objective: To investigate the effect of SOX10 on bladder cancer cells promoting human umbilical vein endothelial cell (HUVEC) proliferation, migration and tube formation. Methods: SOX10 siRNA was transiently transfected into bladder cancer cell line T24 in vitro by Lipofectamine 2000. The expression of SOX10 was examined by Western blot 48 h after transfection. The conditioned media from SOX10-siRNA-transfected T24 cells was collected, and MTT assay, Transwell assay and tube formation assay were used to evaluate the effect of conditioned media on HUVECs proliferation, migration and tube formation. Results: The expression of SOX10 was decreased in T24 cells after SOX10-siRNA transfection. The effect of conditioned media from SXO10-siRNA-transfected T24 cells on proliferation, migration and tube formation of HUVEC was significantly decreased compared with blank control and negative control (P<0.001). Conclusion: SOX10 could influence the HUVECs proliferation, migration and tube formation via paracrine in bladder cancer.
Keywords：
SOX10; bladder cancer; endothelial cells; angiogenesis