多肽pATMtide 在先天性心脏病中的作用
| 作者: |
1冯梦文,
1尹安雯,
1程子杰,
1徐佳,
1章浩,
1张奇军,
1李华,
1钱玲梅
1 南京医科大学第一临床医学院心内科，南京 210029 |
| 通讯: |
钱玲梅
Email: lmqian@njmu.edu.cn |
| DOI: | 10.3978/j.issn.2095-6959.2018.02.001 |
| 基金: | 国家自然科学基金(81370278，81570209)。 |
摘要
目的：研究多肽pATMtide在P19细胞(小鼠畸胎瘤细胞)的分化及斑马鱼心脏发育中的作用，探讨其与先天性心脏病(congenital heart disease，CHD)之间的关系。方法：从已报道的CHD羊水多肽图谱中筛选出1条表达明显上调的多肽pATMtide。在体外实验中，通过加入外源性多肽pATMtide，检测其对P19细胞分化的影响；在体内实验中，将多肽pATMtide通过显微注射技术注入到斑马鱼受精卵，观察其对斑马鱼心脏发育的影响。结果：与对照组相比，加入外源性多肽pATMtide明显抑制了P19细胞的分化，各心脏发育相关标志物表达水平明显下调。斑马鱼实验表明：多肽pATMtide可造成斑马鱼心脏发育畸形。结论：多肽pATMtide可影响P19细胞分化并导致斑马鱼心脏发育畸形，表明多肽pATMtide与CHD的形成有一定的关系。
关键词：
多肽；先天性心脏病；P19细胞；斑马鱼；显微注射
Effect of pATMtide on congenital heart disease
CorrespondingAuthor: QIAN Lingmei Email: lmqian@njmu.edu.cn
DOI: 10.3978/j.issn.2095-6959.2018.02.001
Foundation: This work was supported by the National Natural Science Foundation of China (81370278, 81570209).
Abstract
Objective: To investigate the function of pATMtide in the heart development of zebra fish and differentiation of P19 cells. Methods: An obvious up-regulated polypeptide pATMtide was found from our previous study. In vitro experiment, the effect of pATMtide on P19 cells differentiation were detected; and in vivo experiment, after microinjecting the zygotes zebra fish with pATMtide, the cardiac development of zebra fish was observed.
Results: The differentiation and related cardiac biomarkers of P19 cells are reduced as well as the heart development of zebrafish after adding pATMtide. Conclusion: The differentiation and development are influenced after adding pATMtide. It indicates that pATMtide may play an important role in congenital heart disease.
Keywords：
peptides; congenital heart disease; P19 cells; zebra fish; microinjection
Results: The differentiation and related cardiac biomarkers of P19 cells are reduced as well as the heart development of zebrafish after adding pATMtide. Conclusion: The differentiation and development are influenced after adding pATMtide. It indicates that pATMtide may play an important role in congenital heart disease.