定量检测膀胱尿路上皮癌患者手术前后外周血中survivin mRNA的临床意义
| 作者: |
1陈平锋
1 衡阳市南华大学附属第一医院泌尿外科，湖南 衡阳 421001 |
| 通讯: |
陈平锋
Email: erdonlei@sina.com |
| DOI: | 10.3978/j.issn.2095-6959.2018.01.005 |
| 基金: | 衡阳市科技计划项目(2102KJ50)。 |
摘要
目的：研究膀胱移行细胞癌患者手术前后全血中survivin mRNA表达变化及其临床意义。方法：采用qRT-PCR检测47名非肌层浸润膀胱癌患者与25名正常人群手术前后外周血中survivin mRNA表达量并构建受试者工作特征(receiver operating characteristic，ROC)曲线。结果：术前膀胱癌患者survivin mRNA表达水平是正常人群的4.06倍(P<0.05)，经ROC曲线分析，区别膀胱癌患者与正常人群的survivin表达临界值为1.4×10−2，患者组术后各时间点survivin表达水平明显低于术前水平(P<0.05)，survivin mRNA水平高于临界值的术后患者较低于临界值的患者复发率高(P<0.05)。结论：qRT-PCR可简单敏感地检测全血中survivin mRNA水平，survivin可作为用于膀胱癌诊断及复发判断的标志物。
关键词：
膀胱癌；survivin；外周血；mRNA；qRT-PCR
Clinical significance of survivin mRNA detection in the peripheral whole blood of patients with bladder urothelial carcinoma before and after surgery
CorrespondingAuthor: CHEN Pingfeng Email: erdonlei@sina.com
DOI: 10.3978/j.issn.2095-6959.2018.01.005
Foundation: This work was supported by the Science and Technology Project Foundation of Hengyang, China (2102KJ50).
Abstract
Objective: To investigate the mRNA expression of survivin in whole blood of patients with bladder urothelial carcinoma. Methods: Levels of survivin mRNA expression in the whole blood of 47 patients with non-muscle-invasive bladder cancer before and after operations and 25 normal controls were detected by qRT-PCR and the receiver operating characteristic (ROC) curve for each factor was constructed. Results: The levels of survivin mRNA in bladder cancer patients were 4.06 folds compared to normal controls (P<0.05). The cutoff value for differentiating bladder cancer patients and normal people was 1.4×10−2 for survivin. Postoperative survivin expression in patients was significantly lower than preoperative expression at each time point (P<0.05). Post-operative recurrence rate of post-operative patients with mRNA level above cutoff value were higher than lower ones (P<0.05). Conclusion: qRT-PCR is a convenient and sensitive method to detect survivin mRNA in the whole blood using , and survivin could be useful tumor biomarkers for early detection and recurrence of bladder cancer.
Keywords：
bladder urothelial carcinoma; survivin; peripheral blood; mRNA; qRT-PCR